Isolation, molecular characterization and prevalence of Clostridium perfringens in sheep and goats of Kashmir Himalayas, India
The study was conducted to report the occurrence of the in sheep and goats of the Kashmir valley for the 1 time and to characterize them molecularly with respect to toxin genes to determine the prevalence of the various toxinotypes. A total of 177 samples (152 from sheep and 25 from goats) collected...
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| Published in: | Veterinary World Vol. 10; no. 12; pp. 1501 - 1507 |
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| Main Authors: | , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
India
Veterinary World
01-12-2017
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | The study was conducted to report the occurrence of the
in sheep and goats of the Kashmir valley for the 1
time and to characterize them molecularly with respect to toxin genes to determine the prevalence of the various toxinotypes.
A total of 177 samples (152 from sheep and 25 from goats) collected from healthy, diarrheic animals, and morbid material of animals suspected to have died of enterotoxaemia were screened for
toxinotypes. The presumptive positive isolates were confirmed using 16S rRNA gene-based polymerase chain reaction (PCR). All the confirmed isolates were screened for six toxin genes, namely;
, and
using a multiplex PCR.
The PCR amplification of 16S rRNA gene revealed that out of 177 samples collected, 125 (70.62%) were found positive for
, of which 110 (72.36%) were from sheep and 15 (60%) were from goats. The highest prevalence of
toxinotype D was observed in lambs (56.16%) and kids (46.16%) followed by 3.84% in adult sheep while it was absent in samples obtained from adult goats. The multiplex PCR revealed that 67 (60.90%) isolates from sheep and 8 (53.33%) isolates from goats belonged to toxinotype A, while 43 (39.09%) isolates from sheep and 7 (46.66%) isolates from goats were detected as toxinotype D. None of the isolates was found to be toxinotype B, C, or E. All the
toxinotype A isolates from sheep were negative for both
and
genes, however, 27.90% toxinotype D isolates from sheep carried
gene, and 6.97% possessed
gene. In contrast, 12.50%
toxinotype A isolates from goats harbored
and
genes while 14.28% isolates belonging to toxinotype D carried
and
genes, respectively.
The high prevalence of
was observed, even in day-old lambs. The toxinotypes A and D are prevalent in both sheep and goats. The severity of disease and mortality may be associated with the presence of minor toxins in both the detected toxinotypes. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0972-8988 2231-0916 |
| DOI: | 10.14202/vetworld.2017.1501-1507 |