Comparison of an indirect ELISA with the Brucella milk ring test for detection of antibodies to Brucella abortus in bulk milk samples

An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of Brucella abortus antibodies in bovine bulk milk samples was evaluated. About 31 individual milk samples from B. abortus infected cows were diluted into bulk milk from a brucellosis free herd. Individual milk samples obtained...

Full description

Saved in:

Bibliographic Details
Published in:	Veterinary microbiology Vol. 82; no. 1; pp. 55 - 60
Main Authors:	Vanzini, V.R., Aguirre, N.P., Valentini, B.S., Torioni de Echaide, S., Lugaresi, C.I., Marchesino, M.D., Nielsen, K.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 03-09-2001 Elsevier Science
Subjects:	Animals Antibodies, Bacterial - analysis Argentina Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Brucella abortus Brucella abortus - isolation & purification Bulk milk sample Cattle Cattle-bacteria Dairying Diagnosis-bacteria ELISA Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Female Food Microbiology Fundamental and applied biological sciences. Psychology Fundamental immunology Microbiology Milk - microbiology Molecular immunology Ring test Techniques Ring test Argentina Diagnosis-bacteria Bulk milk sample Cattle-bacteria Brucella abortus ELISA Bovine Antibody Enzyme Ox Cow Infection Vertebrata Dilution Sensitivity Specificity Mammalia Brucellaceae Bacteriosis Bacteria Brucellosis Serum Artiodactyla Detection ELISA assay Ungulata Brucella melitensis Milk
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of Brucella abortus antibodies in bovine bulk milk samples was evaluated. About 31 individual milk samples from B. abortus infected cows were diluted into bulk milk from a brucellosis free herd. Individual milk samples obtained from 96 negative or positive herds to ELISA or Brucella ring test (BRT), were tested by ELISA. All positive cows were bled and serum samples were tested by the complement-fixation test (CFT) which was considered the definitive test. A herd was considered infected if at least, one cow was positive in the CFT. Four samples were negative in the BRT at the dilution 1:10 but positive in the ELISA. For samples positive in both tests, BRT titers ranged from 1:10 to 1:480 while ELISA titers ranged from 1:10 to 1:3200. Using bulk milk samples, the sensitivity of the ELISA (98.1%) was higher than the BRT (72.2%) but the specificity of BRT (90.5%) was not statistically different ( P=1.0) from the ELISA (88.1%). The implications of the results for brucellosis control are discussed.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0378-1135 1873-2542
DOI:	10.1016/S0378-1135(01)00370-4