SlNAC2 overexpression in Arabidopsis results in enhanced abiotic stress tolerance with alteration in glutathione metabolism

SlNAC2 overexpression in Arabidopsis results in enhanced abiotic stress tolerance with alteration in glutathione metabolism

Plant NAC (NAM, ATAF, and CUC) transcription factors (TF) have important roles to play in abiotic stress responses through activation of a battery of functional genes/transcriptional regulators responsible for stress tolerance. Here we report the cloning of a novel Solanum lycopersicum L., NAC2 TF h...

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Bibliographic Details
Published in:Protoplasma Vol. 256; no. 4; pp. 1065 - 1077
Main Authors: Borgohain, Pankaj, Saha, Bedabrata, Agrahari, Rajkishan, Chowardhara, Bhaben, Sahoo, Smita, van der Vyver, Christell, Panda, Sanjib Kumar
Format: Journal Article
Language:English
Published: Vienna Springer Vienna 01-07-2019
Springer Nature B.V
Subjects:
Abiotic stress
Biomedical and Life Sciences
Cell Biology
Drought
Glutathione reductase
Life Sciences
Metabolism
Nucleotides
Original Article
Osmotic stress
Phylogeny
Plant Sciences
Polymerase chain reaction
Reactive oxygen species
Sodium chloride
Transcription factors
Transgenic plants
Zoology
NAC
Tomato
Drought
Abiotic stress
Glutathione
Salinity
Arabidopsis thaliana
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Summary:Plant NAC (NAM, ATAF, and CUC) transcription factors (TF) have important roles to play in abiotic stress responses through activation of a battery of functional genes/transcriptional regulators responsible for stress tolerance. Here we report the cloning of a novel Solanum lycopersicum L., NAC2 TF having 960 nucleotides long CDS (GenBank: KT740994.1). Phylogenetic analysis depicted the similarity of Sl NAC2 to other orthologs. Sl NAC2 was overexpressed in Arabidopsis thaliana to assess and characterize its role in plant abiotic stress responses. The transgenic events were first confirmed by genomic DNA PCR and qRT PCR; then the T3 generation plants were used for stress assays. Soil stress assay depicted better survivability of the transgenic plants under both salt (NaCl) and drought (PEG) stress. The transgenic plants showed enhanced endurance; with better antioxidative response, reduced accumulation of reactive oxygen species (ROS) molecules and better retention of water in tissue. This study for the very first time analyzed the different stakeholders of the glutathione metabolism in Sl NAC2 overexpressing transgenic lines on exposure to both salinity and PEG stress. The expression of the two genes (ɤ-ECS, GS) responsible for glutathione biosynthesis increased with Sl NAC2 overexpression. Further glutathione reductase responsible for reduction of glutathione disulfide (GSSG) to glutathione (GSH) also increased significantly which suggested the regulation of glutathione metabolism as a mechanism for the osmotic stress tolerance conferred to plants upon NAC overexpression.
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ISSN:0033-183X
1615-6102
DOI:10.1007/s00709-019-01368-0