Search Results - "Aggeler, R"

Ligand-dependent structural variations in Escherichia coli F1 ATPase revealed by cryoelectron microscopy by GOGOL, E. P, JOHNSTON, E, AGGELER, R, CAPALDI, R. A

“…The Escherichia coli F1 ATPase, ECF1, has been examined by cryoelectron microscopy after reaction with Fab' fragments generated from monoclonal antibodies to…”
Get full text

Energy substrate modulates mitochondrial structure and oxidative capacity in cancer cells by ROSSIGNOL, Rodrigue, GILKERSON, Robert, AGGELER, Robert, YAMAGATA, Kunihiro, REMINGTON, S. James, CAPALDI, Roderick A

“…Comparative analysis of cytoplasmic organelles in a variety of tumors relative to normal tissues generally reveals a strong diminution in mitochondrial content…”
Get full text

Investigating Mitochondrial Redox Potential with Redox-sensitive Green Fluorescent Protein Indicators by Hanson, George T., Aggeler, Robert, Oglesbee, Devin, Cannon, Mark, Capaldi, Roderick A., Tsien, Roger Y., Remington, S. James

“…Current methods for determining ambient redox potential in cells are labor-intensive and generally require destruction of tissue. This precludes single cell or…”
Get full text

ATP hydrolysis-linked structural changes in the N-terminal part of the gamma subunit of Escherichia coli F1-ATPase examined by cross-linking studies by AGGELER, R, CAPALDI, R. A

“…A mutant of Escherichia coli F1-ATPase (ECF1) in which the serine residue in position 8 of the gamma subunit has been replaced by a cysteine residue (gamma…”
Get full text

Cross-linking of the gamma subunit of the Escherichia coli ATPase (ECF1) via cysteines introduced by site-directed mutagenesis by Aggeler, R, Capaldi, R.A.

“…The gamma subunit of the Escherichia coli F1 ATPase (ECF1) has been altered by site-directed mutagenesis to create five different mutants, gamma-S8C,…”
Get full text

Yeast cytochrome c oxidase subunit VII is essential for assembly of an active enzyme. Cloning, sequencing, and characterization of the nuclear-encoded gene by Aggeler, R, Capaldi, R A

“…The gene COX VII coding for yeast cytochrome c oxidase subunit VII has been cloned by a two-step procedure. Two degenerate oligonucleotides corresponding to…”
Get full text

Mechanism of the F1F0-type ATP synthase, a biological rotary motor by Capaldi, Roderick A, Aggeler, Robert

Get full text

Conformational changes in the gamma and epsilon subunits are integral to the functioning of the Escherichia coli H(+)-pumping ATPase (ECF1F0) by Capaldi, R A, Aggeler, R, Wilkens, S

“…ATP synthesis and ATP hydrolysis by F1F0-type ATPases involve conformational changes transmitted from the catalytic site regions to the proton channel, a…”
Get more information

Cryoelectron microscopy of Escherichia coli F1 adenosinetriphosphatase decorated with monoclonal antibodies to individual subunits of the complex by Gogol, Edward P, Aggeler, R, Sagermann, M, Capaldi, Roderick A

“…Monoclonal antibodies directed against epitopes on each of the five subunits (alpha, beta, gamma, delta, and epsilon) of the Escherichia coli F1 ATPase (ECF1)…”
Get full text

The cysteine introduced into the alpha subunit of the Escherichia coli F1-ATPase by the mutation alpha R376C is near the alpha-beta subunit interface and close to a noncatalytic nucleotide binding site by TURINA, P, AGGELER, R, LEE, R. S. F, SENIOR, A. E, CAPALDI, R. A

“…Mutation of the alpha subunit of the Escherichia coli F1-ATPase to convert Arg-376 to a Cys (alpha R376C) lowers multisite ATPase activity 400-1,000-fold while…”
Get full text

Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays by Nadanaciva, Sashi, Bernal, Autumn, Aggeler, Robert, Capaldi, Roderick, Will, Yvonne

“…Mitochondrial dysfunction has been shown to be a pharmacotoxicological response to a variety of currently-marketed drugs. In order to reduce attrition due to…”
Get full text

Isolation and characterization of the cDNAs encoding two isoforms of subunit CIX of bovine cytochrome c oxidase by LIGHTOWLERS, R, EWART, G, AGGELER, R, YU-ZHONG ZHANG, CALAVETTA, L, CAPALDI, R. A

“…The smallest subunit of bovine cytochrome c oxidase (CIX or VIII in different nomenclatures) occurs in two isoforms, a heart (H) form and a liver (L) form. The…”
Get full text

Analysis of Steady-state Protein Phosphorylation in Mitochondria Using a Novel Fluorescent Phosphosensor Dye by Schulenberg, Birte, Aggeler, Robert, Beechem, Joseph M., Capaldi, Roderick A., Patton, Wayne F.

“…The phosphorylation of mitochondrial proteins is pivotal to the regulation of respiratory activity in the cell and to signaling pathways leading to apoptosis,…”
Get full text

Characterization of dynamic and steady-state protein phosphorylation using a fluorescent phosphoprotein gel stain and mass spectrometry by Schulenberg, Birte, Goodman, Terrie N., Aggeler, Robert, Capaldi, Roderick A., Patton, Wayne F.

“…Protein phosphorylation plays a vital role in the regulation of most aspects of cellular activity, being key to propagating messages within signal transduction…”
Get full text

Large conformational changes of the epsilon subunit in the bacterial F1F0 ATP synthase provide a ratchet action to regulate this rotary motor enzyme by Tsunoda, S P, Rodgers, A J, Aggeler, R, Wilce, M C, Yoshida, M, Capaldi, R A

“…The F(1)F(0) ATP synthase is the smallest motor enzyme known. Previous studies had established that the central stalk, made of the gamma and epsilon subunits…”
Get full text

TFPACD, a novel bifunctional reagent for reacting with DCCD sites in proteins: studies using Escherichia coli ATP synthase by Phadke, A S, Aggeler, R, Keana, J F, Capaldi, R A

“…A novel cross-linker, 1-[6-(4-azido-2,3,5,6-tetrafluorobenzamido)hexyl]-3-cyclohexylc arbodiimide (TFPACD), has been synthesized and tested by reaction with…”
Get more information

Epitope mapping of monoclonal antibodies to the Escherichia coli F1 ATPase alpha subunit in relation to activity effects and location in the enzyme complex based on cryoelectron microscopy by Aggeler, R, Capaldi, R A, Dunn, S, Gogol, E P

“…The interaction of Escherichia coli F1 ATPase (ECF1) with several different monoclonal antibodies (mAbs) specific for the alpha subunit has been examined. The…”
Get more information

Mass spectrometric identification of a novel phosphorylation site in subunit NDUFA10 of bovine mitochondrial complex I by Schilling, Birgit, Aggeler, Robert, Schulenberg, Birte, Murray, James, Row, Richard H., Capaldi, Roderick A., Gibson, Bradford W.

“…Mitochondrial Complex I (NADH:ubiquinone oxidoreductase) consists of at least 46 subunits. Phosphorylation of the 42-kDa subunit NDUFA10 was recently reported…”
Get full text

Cross-linking of the δ Subunit to One of the Three α Subunits Has No Effect on Functioning, as Expected if δ Is a Part of the Stator That Links the F1 and F0 Parts of the Escherichia coli ATP Synthase by Ogilvie, Isla, Aggeler, Robert, Capaldi, Roderick A

“…A mutant of the Escherichia coliF1F0-ATPase has been generated (αQ2C) in which the glutamine at position 2 of the α subunit has been replaced with a cysteine…”
Get full text

Labeling of the ATP synthase of Escherichia coli from the head-group region of the lipid bilayer by Aggeler, Robert, Zhang, Yu Zhong, Capaldi, Roderick A

“…The isolated and membrane-bound forms of the adenosinetriphosphatase of Escherichia coli (ECF1 and ECF1F0, respectively) have been reacted with two…”
Get full text