Search Results - "Agasid, Mark T."

Membrane Protein-Lipid Interactions Probed Using Mass Spectrometry by Bolla, Jani Reddy, Agasid, Mark T, Mehmood, Shahid, Robinson, Carol V

“…Membrane proteins that exist in lipid bilayers are not isolated molecular entities. The lipid molecules that surround them play crucial roles in maintaining…”
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Decreased Aperture Surface Energy Enhances Electrical, Mechanical, and Temporal Stability of Suspended Lipid Membranes by Bright, Leonard K, Baker, Christopher A, Agasid, Mark T, Ma, Lin, Aspinwall, Craig A

“…The development of next-generation transmembrane protein-based biosensors relies heavily on the use of black lipid membranes (BLMs); however, electrical,…”
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Probing membrane protein–lipid interactions by Agasid, Mark T., Robinson, Carol V.

“…Structure determination of membrane proteins has highlighted the many roles played by lipids in influencing overall protein architecture. It is now widely…”
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The Effects of Sodium Ions on Ligand Binding and Conformational States of G Protein-Coupled ReceptorsInsights from Mass Spectrometry by Agasid, Mark T, Sørensen, Lars, Urner, Leonhard H, Yan, Jun, Robinson, Carol V

“…The use of mass spectrometry to investigate proteins is now well established and provides invaluable information for both soluble and membrane protein…”
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Surface Modification of Glass/PDMS Microfluidic Valve Assemblies Enhances Valve Electrical Resistance by Wang, Xuemin, Agasid, Mark T, Baker, Christopher A, Aspinwall, Craig A

“…Microfluidic instrumentation offers unique advantages in biotechnology applications including reduced sample and reagent consumption, rapid mixing and reaction…”
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Native Protein Mass Spectrometry by Allison, Timothy M, Agasid, Mark T

“…In native mass spectrometry, non-covalent interactions are preserved in solution and through transfer to the gas phase. This technique can be used to…”
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Detergents with Scalable Properties Identify Noncanonical Lipopolysaccharide Binding to Bacterial Inner Membrane Proteins by Urner, Leonhard H., Fiorentino, Francesco, Shutin, Denis, Sauer, Joshua B., Agasid, Mark T., El-Baba, Tarick J., Bolla, Jani R., Stansfeld, Phillip J., Robinson, Carol V.

“…Lipopolysaccharide (LPS) is vital for maintaining the outer membrane barrier in Gram-negative bacteria. LPS is also frequently obtained in complex with the…”
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Enhanced Temporal Resolution with Ion Channel-Functionalized Sensors Using a Conductance-Based Measurement Protocol by Agasid, Mark T, Comi, Troy J, Saavedra, S. Scott, Aspinwall, Craig A

“…The binding of a target analyte to an ion channel (IC), which is readily detected electrochemically in a label-free manner with single-molecule selectivity and…”
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Mass-selective and ice-free electron cryomicroscopy protein sample preparation via native electrospray ion-beam deposition by Esser, Tim K, Böhning, Jan, Fremdling, Paul, Agasid, Mark T, Costin, Adam, Fort, Kyle, Konijnenberg, Albert, Gilbert, Joshua D, Bahm, Alan, Makarov, Alexander, Robinson, Carol V, Benesch, Justin L P, Baker, Lindsay, Bharat, Tanmay A M, Gault, Joseph, Rauschenbach, Stephan

“…Despite tremendous advances in sample preparation and classification algorithms for electron cryomicroscopy (cryo-EM) and single-particle analysis (SPA),…”
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Electrospray ionization of native membrane proteins proceeds via a charge equilibration step by Yen, Hsin-Yung, Abramsson, Mia L, Agasid, Mark T, Lama, Dilraj, Gault, Joseph, Liko, Idlir, Kaldmäe, Margit, Saluri, Mihkel, Qureshi, Abdul Aziz, Suades, Albert, Drew, David, Degiacomi, Matteo T, Marklund, Erik G, Allison, Timothy M, Robinson, Carol V, Landreh, Michael

“…Electrospray ionization mass spectrometry is increasingly applied to study the structures and interactions of membrane protein complexes. However, the charging…”
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Expression, purification, and electrophysiological characterization of a recombinant, fluorescent Kir6.2 in mammalian cells by Agasid, Mark T., Wang, Xuemin, Huang, Yiding, Janczak, Colleen M., Bränström, Robert, Saavedra, S. Scott, Aspinwall, Craig A.

“…The inwardly rectifying K+ (Kir) channel, Kir6.2, plays critical roles in physiological processes in the brain, heart, and pancreas. Although Kir6.2 has been…”
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Investigating Protein-Lipid Interactions of MthK and Native Bacterial Membrane Lipids by Quetschlich, Daniel, Agasid, Mark T., Davis, Rachel A., Sands, Zara A., Liko, Idlir, Sansom, Mark S., Stansfeld, Phillip J., Robinson, Carol V.

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Enhanced temporal resolution with ion channel-functionalized sensors using rapid conductance measurement by Agasid, Mark T., Comi, Troy J., Saavedra, S. Scott, Aspinwall, Craig A.

“…The binding of a target analyte to an ion channel (IC), which is readily detected electrochemically in a label-free manner with single-molecule selectivity and…”
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Electrospray ionization of native membrane proteins proceeds a charge equilibration step by Yen, Hsin-Yung, Abramsson, Mia L, Agasid, Mark T, Lama, Dilraj, Gault, Joseph, Liko, Idlir, Kaldmäe, Margit, Saluri, Mihkel, Qureshi, Abdul Aziz, Suades, Albert, Drew, David, Degiacomi, Matteo T, Marklund, Erik G, Allison, Timothy M, Robinson, Carol V, Landreh, Michael

“…Electrospray ionization mass spectrometry is increasingly applied to study the structures and interactions of membrane protein complexes. However, the charging…”
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I. Development of rapid conductance-based protocols for measuring ion channel activity. II. Expression, characterization, and purification of the ATP-sensitive, inwardly-rectifying K+-channel, Kir6.2 and ion channel-coupled receptors by Agasid, Mark T

“…Ligand-gated and ligand-modulated ion channel (IC) sensors have received increased attention for their ability to transduce ligand-binding events into a…”
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