A Rapid Derivative Spectrophotometric Method for Simultaneous Determination of Naphazoline and Antazoline in Eye Drops

A Rapid Derivative Spectrophotometric Method for Simultaneous Determination of Naphazoline and Antazoline in Eye Drops

A zero-crossing first-derivative spectrophotometric method is applied for the simultaneous determination of naphazoline hydrochloride and antazoline phosphate in eye drops. The measurements were carried out at wavelengths of 225 and 252 nm for naphazoline hydrochloride and antazoline phosphate, resp...

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Bibliographic Details
Published in:Chemical & Pharmaceutical Bulletin Vol. 54; no. 1; pp. 119 - 122
Main Authors: Souri, Effat, Amanlou, Massoud, Farsam, Hassan, Afshari, Alma
Format: Journal Article
Language:English
Published: Japan The Pharmaceutical Society of Japan 2006
Pharmaceutical Society of Japan
Japan Science and Technology Agency
Subjects:
Adrenergic alpha-Agonists - analysis
Antazoline - analysis
antazoline phosphate
Anti-Allergic Agents - analysis
Calibration
derivative spectrophotometry
eye drops
Indicators and Reagents
Naphazoline - analysis
naphazoline hydrochloride
Ophthalmic Solutions - analysis
Reference Standards
Reproducibility of Results
Spectrophotometry, Ultraviolet
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Summary:A zero-crossing first-derivative spectrophotometric method is applied for the simultaneous determination of naphazoline hydrochloride and antazoline phosphate in eye drops. The measurements were carried out at wavelengths of 225 and 252 nm for naphazoline hydrochloride and antazoline phosphate, respectively. The method was found to be linear (r2>0.999) in the range of 0.2—1 μg/ml for naphazoline hydrochloride in the presence of 5 μg/ml antazoline phosphate at 225 nm. The same linear correlation (r2>0.999) was obtained in the range of 1—10 μg/ml of antazoline phosphate in the presence of 0.5 μg/ml of naphazoline hydrochloride at 252 nm. The limit of determination was 0.2 μg/ml and 1 μg/ml for naphazoline hydrochloride and antazoline phosphate, respectively. The method was successfully used for simultaneous analysis of naphazoline hydrochloride and antazoline phosphate in eye drops without any interference from excipients and prior separation before analysis.
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ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.54.119