Epidemiology and genetic characterization of human sapovirus among hospitalized acute diarrhea patients in Bangladesh, 2012–2015

Epidemiology and genetic characterization of human sapovirus among hospitalized acute diarrhea patients in Bangladesh, 2012–2015

Human sapovirus, which causes acute gastroenteritis, is not well studied and poorly understood. This study aims to investigate the contribution of sapovirus in diarrhea, their clinical association, and genotypic diversity. Fecal specimens (n = 871) were randomly selected from diarrheal patients who...

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Bibliographic Details
Published in:Journal of medical virology Vol. 93; no. 11; pp. 6220 - 6228
Main Authors: Rahman, Rajibur, Rahman, Sezanur, Afrad, Md M. H., Talha, Muhammad, Islam, Deen, Uddin, K. M. Main, Ahmed, Shahnawaz, Afrad, Mokibul H., Faruque, Abu S. G., Hossian, Mohammad E., Rahman, Mustafizur
Format: Journal Article
Language:English
Published: United States Wiley Subscription Services, Inc 01-11-2021
Subjects:
Acute Disease
Adolescent
Adult
Bangladesh - epidemiology
Caliciviridae Infections - epidemiology
Caliciviridae Infections - virology
Child
Child, Preschool
Diarrhea
Diarrhea - virology
DNA-directed RNA polymerase
E coli
Epidemiology
Feces - virology
Female
Gastroenteritis
Gastroenteritis - epidemiology
Gastroenteritis - virology
Genetic diversity
Genetic Variation
Genotype
Genotypes
genotypic characterization
hospitalized acute diarrhea
Humans
Infant
Infant, Newborn
Male
molecular epidemiology
Patients
Polymerase chain reaction
Real-Time Polymerase Chain Reaction
Ribonucleic acid
RNA
Rotavirus
sapovirus
Sapovirus - genetics
Strains (organisms)
Virology
Young Adult
Bangladesh
molecular epidemiology
genotypic characterization
hospitalized acute diarrhea
sapovirus
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Summary:Human sapovirus, which causes acute gastroenteritis, is not well studied and poorly understood. This study aims to investigate the contribution of sapovirus in diarrhea, their clinical association, and genotypic diversity. Fecal specimens (n = 871) were randomly selected from diarrheal patients who attended International Centre for Diarrhoeal Disease Research, Bangladesh hospital in Dhaka, Bangladesh during January 2012–December 2015 and tested for the presence of sapovirus RNA using real‐time polymerase chain reaction. Sapovirus RNA was identified in 2.3% (n = 20) of the samples. Seventy‐five percent of the sapovirus positive cases were coinfected with other pathogens, such as rotavirus, norovirus, enterotoxigenic Escherichia coli, adenovirus, Shigella spp., and Vibrio cholerae. A vast genetic diversity was observed among sapovirus with at least seven common genotypes (GI.1, GI.2, GI.7, GII.1, GII.4, GII.6, and GIV), and a new genotype GII.NA1. Some of the GI.1 strains detected were similar to GI.4 in the polymerase region sequence and were confirmed as recombinant strains. Our findings suggest that the overall contribution of sapovirus in hospitalized diarrheal illness is low but highlight enormous genetic diversity. Highlights The findings of this study provide a new insight into the extent of co‐infections and genetic diversity of sapovirus.
Bibliography:Rajibur Rahman and Sezanur Rahman contributed equally to this study and are considered as first co‐authors.
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ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.27125