Heterogeneous inbred populations are useful as sources of near-isogenic lines for RAPD marker localization

The development and use of RAPD markers for applications in crop improvement has recently generated considerable interest within the plant breeding community. One potential application of RAPDs is their use for "tagging" simply-inherited (monogenic) pest-resistance genes and enabling more...

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Bibliographic Details
Published in:	Theoretical and applied genetics Vol. 88; no. 3-4; pp. 337 - 342
Main Authors:	Haley, S.D, Afanador, L.K, Miklas, P.N, Stavely, J.R, Kelly, J.D. (Michigan State Univ., East Lansing (USA). Dept. of Crop and Soil Sciences)
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer 01-06-1994 Berlin
Subjects:	Biological and medical sciences Classical genetics, quantitative genetics, hybrids Fundamental and applied biological sciences. Psychology GENE GENES Genetics of eukaryotes. Biological and molecular evolution LIGNEE CONSANGUINE LINEAS CONSANGUINEAS MARCADORES GENETICOS MARQUEUR GENETIQUE PHASEOLUS VULGARIS POLIMORFISMO GENETICO POLYMORPHISME GENETIQUE Pteridophyta, spermatophyta RESISTANCE AUX MALADIES RESISTENCIA A LA ENFERMEDAD ROUILLE ROYA UROMYCES Uromyces appendiculatus Vegetals Performance evaluation Genetic selection Linkage Genetic marker Uromyces appendiculatus Basidiomycetes Fungus resistance Fungi Leguminosae Random amplified polymorphic DNA Phaseolus vulgaris Dicotyledones Angiospermae Spermatophyta Isogenic line Thallophyta Polymorphism
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Summary:	The development and use of RAPD markers for applications in crop improvement has recently generated considerable interest within the plant breeding community. One potential application of RAPDs is their use for "tagging" simply-inherited (monogenic) pest-resistance genes and enabling more efficient identification and selection of genotypes carrying specific combinations of resistance genes. In this report, the use of heterogeneous inbred populations as sources of near-isogenic lines (NILs) is proposed for targeting RAPD markers linked to major pest resistance genes. The development of these NILs for RAPD marker analyses involved a sequence of line and mass selection during successive generations of inbreeding. DNA bulks derived from the NILs were used to identify a RAPD marker (designated OK14(620), generated by 5'CCCGCTACAC-3' decamer) that was tightly linked (2.23 +-1.33 centi Morgans) to an important rust [Uromyces appendiculatus (Pers.) Unger var. appendiculatus] resistance gene (Ur-3) in common bean (Phaseolus vulgaris L.). The efficiency of this approach was demonstrated by a low rate of false-positives identified, the tightness of the linkage identified, and the ability to detect polymorphism between genomic regions that are representative of the same gene pool of common bean. This method of deriving NILs should find application by researchers interested in utilizing marker-assisted selection for one or more major pest resistance genes. The identification of OK14(620) should help to facilitate continued use of the Ur-3 resistance source and will now enable marker-assisted pyramiding of three different bean rust resistance sources (two previously tagged) to provide effective and stable resistance to this important pathogen.
Bibliography:	97B7469 F30 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0040-5752 1432-2242
DOI:	10.1007/BF00223642