Relevance of autoantibody detection to the rapid diagnosis of brucellosis

Abstract The rose bengal test is often used for rapid diagnosis of human brucellosis in endemic areas. However, autoantibodies have never been investigated as a reason for false-positive or false-negative results. Therefore, the aim of this study was to show the effect of autoantibody detection on t...

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Published in:	Diagnostic microbiology and infectious disease Vol. 58; no. 3; pp. 271 - 273
Main Authors:	Yumuk, Zeki, Afacan, Gokan, Çalışkan, Şeyda, Irvem, Arzu, Arslan, Ugurgul
Format:	Journal Article
Language:	English
Published:	New York, NY Elsevier Inc 01-07-2007 Elsevier
Subjects:	Agglutination test Antibodies, Antinuclear - blood Antinuclear antibody Autoantibodies - blood Autoantibody Bacterial diseases Biological and medical sciences Brucella Brucella - isolation & purification Brucellosis Brucellosis - diagnosis Brucellosis - immunology Endemic Diseases Human bacterial diseases Humans Infectious Disease Infectious diseases Internal Medicine Medical sciences Miscellaneous Predictive Value of Tests Rose Bengal Sensitivity Sensitivity and Specificity Specificity Statistics as Topic Autoantibody Brucellosis Agglutination test Sensitivity Specificity Antinuclear antibody Infection Autoimmunity Antibody Bacteriosis Diagnosis Detection
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Summary:	Abstract The rose bengal test is often used for rapid diagnosis of human brucellosis in endemic areas. However, autoantibodies have never been investigated as a reason for false-positive or false-negative results. Therefore, the aim of this study was to show the effect of autoantibody detection on the rapid diagnosis of human brucellosis in an endemic area. The study included 2 groups: antinuclear antibody (ANA)-positive and ANA-negative groups. Diagnosis of brucellosis was established by isolation of Brucella spp. from blood culture. The overall sensitivity and specificity of the rose bengal test were 100% and 90.8%, respectively. The specificity (100% versus 89%) and positive predictive value of the test (100% versus 8%) fell markedly from the ANA-negative to the ANA-positive group. As a conclusion, this study verified our suspicion about the effect of autoantibodies on rose bengal test results to the diagnosis of human brucellosis. However, to have definite decisions, extensive studies with larger populations are needed.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2007.01.003