Search Results - "Adriano Gennari"

Effect of by-products from the dairy industry as alternative inducers of recombinant β-galactosidase expression by Mobayed, Francielle Herrmann, Nunes, Juliane Carraro, Gennari, Adriano, de Andrade, Bruna Coelho, Ferreira, Matheus Loch Velvites, Pauli, Paolla, Renard, Gaby, Chies, Jocelei Maria, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…Objective The aim of the present study was to evaluate the efficiency of lactose derived from cheese whey and cheese whey permeate as inducer of recombinant…”
Get full text

Lactose Hydrolysis in Milk and Dairy Whey Using Microbial β-Galactosidases by Dutra Rosolen, Michele, Gennari, Adriano, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…This work aimed at evaluating the influence of enzyme concentration, temperature, and reaction time in the lactose hydrolysis process in milk, cheese whey, and…”
Get full text

A systematic review about affinity tags for one-step purification and immobilization of recombinant proteins: integrated bioprocesses aiming both economic and environmental sustainability by Leonhardt, Fernanda, Gennari, Adriano, Paludo, Graziela Barbosa, Schmitz, Caroline, da Silveira, Filipe Xerxeneski, Moura, Débora Cristina Daenecke Albuquerque, Renard, Gaby, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…The present study reviewed and discussed the promising affinity tags for one-step purification and immobilization of recombinant proteins. The approach used to…”
Get full text

Production Process Optimization of Recombinant Erwinia carotovora l‑Asparaginase II in Escherichia coli Fed-Batch Cultures and Analysis of Antileukemic Potential by de Andrade, Bruna Coelho, Renard, Gaby, Gennari, Adriano, Artico, Leonardo Luís, Júnior, José Ricardo Teixeira, Kuhn, Daniel, Salles, Priscila Pini Zenatti, Volken de Souza, Claucia Fernada, Roth, Gustavo, Chies, Jocelei Maria, Yunes, José Andrés, Basso, Luiz Augusto

“…The aims of this work were to optimize the production of Erwinia carotovora l-asparaginase II enzyme in Escherichia coli by different fed-batch cultivation…”
Get full text

Making a fresh cheese using the colostrum surplus of dairy farms: an alternative aiming to minimize the waste of this raw material by Simon, Renate, Gennari, Adriano, Kuhn, Daniel, Rama, Gabriela Rabaioli, de Souza, Claucia Fernanda Volken

“…Este trabalho teve como objetivo a elaboraçao e caracterizaçao de um queijo fresco a partir do excedente de colostro bovino de fazendas leiteiras. O colostro…”
Get full text

Encapsulation of Lactobacillus spp. using bovine and buffalo cheese whey and their application in orange juice by Rama, Gabriela Rabaioli, Führ, Ana Júlia, da Silva, Jéssica Aparecida Bressan Soratto, Gennari, Adriano, Giroldi, Maiara, Goettert, Márcia Inês, Volken de Souza, Claucia Fernanda

“…The aim of this study was to evaluate and compare the efficiency of bovine (CW) and buffalo cheese whey (BCW) as encapsulating agents for the spray-drying (SD)…”
Get full text

Magnetic cellulose: Versatile support for enzyme immobilization - A review by Gennari, Adriano, Führ, Ana Júlia, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…•Magnetic nanopolymers show suitable attributes as support for immobilized enzymes.•Magnetic cellulose is a versatile support for enzyme…”
Get full text

Recombinant production in Escherichia coli of a β-galactosidase fused to a cellulose-binding domain using low-cost inducers in fed-batch cultivation by Gennari, Adriano, Simon, Renate, de Andrade, Bruna Coelho, Kuhn, Daniel, Renard, Gaby, Chies, Jocelei Maria, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…This study aimed to determine the effects of different feeding strategies in fed-batch cultivations on the production of β-galactosidase fused to a…”
Get full text

Magnetic core-shell cellulose system for the oriented immobilization of a recombinant β-galactosidase with a protein tag by Gennari, Adriano, Simon, Renate, Benvenutti, Edilson Valmir, Nicolodi, Sabrina, Renard, Gaby, Chies, Jocelei Maria, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…The objective of this study was to immobilize a recombinant β-galactosidase (Gal) tagged with a cellulose-binding domain (CBD) onto a magnetic core-shell (CS)…”
Get full text

Nickel-Functionalized Chitosan for the Oriented Immobilization of Histidine-Tagged Enzymes: A Promising Support for Food Bioprocess Applications by de Andrade, Bruna Coelho, Gennari, Adriano, Renard, Gaby, Benvenutti, Edilson Valmir, Chies, Jocelei Maria, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…Recombinant protein biosynthesis and oriented immobilization of enzymes can expand the possibilities of applying biocatalysts to industrial processes. Using…”
Get full text

Production of beta-galactosidase fused to a cellulose-binding domain for application in sustainable industrial processes by Gennari, Adriano, Simon, Renate, de Andrade, Bruna Coelho, Saraiva Macedo Timmers, Luís Fernando, Milani Martins, Vera Lúcia, Renard, Gaby, Chies, Jocelei Maria, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…[Display omitted] •Kluyveromyces sp. β-galactosidase gene was cloned into pET-35b(+) expression vector.•β-Galactosidase-CBD was produced at shaker-incubator…”
Get full text

One-step purification of a recombinant beta-galactosidase using magnetic cellulose as a support: Rapid immobilization and high thermal stability by Gennari, Adriano, Simon, Renate, Sperotto, Nathalia Denise de Moura, Bizarro, Cristiano Valim, Basso, Luiz Augusto, Machado, Pablo, Benvenutti, Edilson Valmir, Da Cas Viegas, Alexandre, Nicolodi, Sabrina, Renard, Gaby, Chies, Jocelei Maria, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…[Display omitted] •One-step purification and targeted immobilization process of a β-galactosidase.•These bioprocesses were carried out efficiently on the…”
Get full text

Synthesis of magnetic nanoparticles functionalized with histidine and nickel to immobilize His-tagged enzymes using β-galactosidase as a model by de Andrade, Bruna Coelho, Gennari, Adriano, Renard, Gaby, Nervis, Brenda Da Rolt, Benvenutti, Edilson Valmir, Costa, Tania Maria Haas, Nicolodi, Sabrina, da Silveira, Nádya Pesce, Chies, Jocelei Maria, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…The aim of this study was to synthesize iron magnetic nanoparticles functionalized with histidine and nickel (Fe3O4-His-Ni) to be used as support materials for…”
Get full text

Promising biocatalysts for producing lactose-free food matrices: β-galactosidase immobilized on cellulose-rich agro-industrial waste supports by Gennari, Adriano, Leonhardt, Fernanda, Tupuna-Yerovi, Diego Santiago, Benvenutti, Edilson Valmir, Nicolodi, Sabrina, Renard, Gaby, Chies, Jocelei Maria, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…This study aimed to develop, characterize, and apply wheat husk and rice husk-based supports for immobilizing β-galactosidase (β-gal) tagged with a Cellulose…”
Get full text

Lactose hydrolysis in packed-and fluidized-bed reactors using a recombinant β-galactosidase immobilized on magnetic core-shell capsules by Gennari, Adriano, Simon, Renate, Renard, Gaby, Chies, Jocelei Maria, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…The objective of this study was to develop a bioprocess for lactose hydrolysis in diverse dairy matrices, specifically skim milk and cheese whey, utilizing…”
Get full text

Application of cellulosic materials as supports for single-step purification and immobilization of a recombinant β-galactosidase via cellulose-binding domain by Gennari, Adriano, Simon, Renate, Sperotto, Nathalia Denise de Moura, Bizarro, Cristiano Valim, Basso, Luiz Augusto, Machado, Pablo, Benvenutti, Edilson Valmir, Renard, Gaby, Chies, Jocelei Maria, Volpato, Giandra, Volken de Souza, Claucia Fernanda

“…This study aimed to develop single-step purification and immobilization processes on cellulosic supports of β-galactosidase from Kluyveromyces sp. combined…”
Get full text

Chelation by collagen in the immobilization of Aspergillus oryzae β-galactosidase: A potential biocatalyst to hydrolyze lactose by batch processes by Gennari, Adriano, Mobayed, Francielle Herrmann, Volpato, Giandra, de Souza, Claucia Fernanda Volken

“…This work is the first study of the immobilization of Aspergillus oryzae β-galactosidase (Gal) on powdered collagen (Col) that had formed a chelate with…”
Get full text

Immobilization of β‑Galactosidases on Magnetic Nanocellulose: Textural, Morphological, Magnetic, and Catalytic Properties by Gennari, Adriano, Mobayed, Francielle H, Da Rolt Nervis, Brenda, Benvenutti, Edilson V, Nicolodi, Sabrina, da Silveira, Nádya Pesce, Volpato, Giandra, Volken de Souza, Claucia F

“…We describe a process for obtaining nanocrystalline cellulose (NC) by either acidic (H–NC) or alkaline treatment (OH–NC) of microcrystalline cellulose, which…”
Get full text

Milk fermentation with prebiotic flour of Vasconcellea quercifolia A.St.-Hil by de Fátima Ferreira da Silva, Lilian, Rodrigues, Kétlin Fernanda, Gennari, Adriano, Ethur, Eduardo Miranda, Hoehne, Lucélia, de Souza, Claucia Fernanda Volken, de Freitas, Elisete Maria

“…Non-conventional food plants have bioactive compounds and a high nutritional value. Among these, Vasconcellea quercifolia has nutritional benefits, but it is…”
Get full text

Modification of Immobead 150 support for protein immobilization: Effects on the properties of immobilized Aspergillus oryzae β‐galactosidase by Gennari, Adriano, Mobayed, Francielle H., da Silva Rafael, Ruan, Rodrigues, Rafael C., Sperotto, Raul A., Volpato, Giandra, Volken de Souza, Claucia F.

“…We studied the modification of Immobead 150 support by either introducing aldehyde groups using glutaraldehyde (Immobead‐Glu) or carboxyl groups through acid…”
Get full text