Search Results - "Adolf, Frank"
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Proteomic Profiling of Mammalian COPII and COPI Vesicles
Published in Cell reports (Cambridge) (02-01-2019)“…Intracellular transport and homeostasis of the endomembrane system in eukaryotic cells depend on the formation and fusion of vesicular carriers. Coat protein…”
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Sec24C/D-isoform-specific sorting of the preassembled ER-Golgi Q-SNARE complex
Published in Molecular biology of the cell (01-09-2016)“…Secretory proteins are exported from the endoplasmic reticulum in COPII vesicles. SNARE proteins-core machinery for membrane fusion-are incorporated into COPII…”
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Membrane curvature induced by Arf1-GTP is essential for vesicle formation
Published in Proceedings of the National Academy of Sciences - PNAS (19-08-2008)“…The GTPase Arf1 is considered as a molecular switch that regulates binding and release of coat proteins that polymerize on membranes to form transport…”
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A novel transglutaminase substrate from Streptomyces mobaraensis triggers autolysis of neutral metalloproteases
Published in Bioscience, biotechnology, and biochemistry (01-05-2009)“…Transglutaminase (TGase) from Streptomyces mobaraensis is a Ca 2+ independent enzyme that cross-links proteins to high molecular weight aggregates. A dispase…”
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COPI budding within the Golgi stack
Published in Cold Spring Harbor perspectives in biology (01-11-2011)“…The Golgi serves as a hub for intracellular membrane traffic in the eukaryotic cell. Transport within the early secretory pathway, that is within the Golgi and…”
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Visualizing chaperone-mediated multistep assembly of the human 20S proteasome
Published in Nature structural & molecular biology (01-08-2024)“…Dedicated assembly factors orchestrate the stepwise production of many molecular machines, including the 28-subunit proteasome core particle (CP) that mediates…”
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PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems
Published in EMBO reports (03-08-2023)“…The protein kinase PINK1 and ubiquitin ligase Parkin promote removal of damaged mitochondria via a feed‐forward mechanism involving ubiquitin (Ub)…”
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Chapter 1 - Analysis of Golgi Complex Functions: In Vitro Reconstitution Systems
Published in Methods in cell biology (01-01-2013)“…In vitro reconstitution is prerequisite to investigate complex cellular functions at the molecular level. Reconstitution systems range from combining complete…”
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Periphilin self-association underpins epigenetic silencing by the HUSH complex
Published in Nucleic acids research (09-10-2020)“…Transcription of integrated DNA from viruses or transposable elements is tightly regulated to prevent pathogenesis. The Human Silencing Hub (HUSH), composed of…”
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Scission of COPI and COPII Vesicles Is Independent of GTP Hydrolysis
Published in Traffic (Copenhagen, Denmark) (01-08-2013)“…Synopsis The question as to whether GTP is hydrolyzed for the scission of a coated bud to release a transport vesicle from a donor membrane is pivotal for…”
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Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission
Published in The Journal of cell biology (05-09-2011)“…Formation of coated vesicles requires two striking manipulations of the lipid bilayer. First, membrane curvature is induced to drive bud formation. Second, a…”
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Targeting of Nbp1 to the inner nuclear membrane is essential for spindle pole body duplication
Published in The EMBO journal (17-08-2011)“…Spindle pole bodies (SPBs), like nuclear pore complexes, are embedded in the nuclear envelope (NE) at sites of fusion of the inner and outer nuclear membranes…”
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ArfGAP1 Activity and COPI Vesicle Biogenesis
Published in Traffic (Copenhagen, Denmark) (01-03-2009)“…Golgi-derived coat protein I (COPI) vesicles mediate transport in the early secretory pathway. The minimal machinery required for COPI vesicle formation from…”
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Analysis of Golgi complex functions: in vitro reconstitution systems
Published in Methods in cell biology (2013)“…In vitro reconstitution is prerequisite to investigate complex cellular functions at the molecular level. Reconstitution systems range from combining complete…”
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The transglutaminase activating metalloprotease inhibitor from Streptomyces mobaraensis is a glutamine and lysine donor substrate of the intrinsic transglutaminase
Published in FEBS letters (03-09-2008)“…Transglutaminase (TGase) from Streptomyces mobaraensis is an extra-cellular enzyme that cross-links proteins to high molecular weight aggregates. Screening for…”
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Targeting of Nbp1 to the inner nuclear membrane is essential for spindle pole body duplication: Targeting of Nbp1 to the inner nuclear membrane
Published in The EMBO journal (17-08-2011)Get full text
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Testing and calibration of coordinate measuring arms
Published in Precision engineering (01-04-2001)“…Portable coordinate measuring arms with rotational axes have found their place in many industrial areas. To establish and confirm the accuracy of these…”
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Analysis of GOLGI Complex Functions: In Vitro Reconstitution Systems
Published in Methods in Cell Biology (2013)Get full text
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Analysis of Golgi Complex Functions
Published in Methods in Cell Biology (2013)“…In vitro reconstitution is prerequisite to investigate complex cellular functions at the molecular level. Reconstitution systems range from combining complete…”
Get full text
Book Chapter