Molecular determination of van genes among clinical isolates of enterococci at a hospital setting

Vancomycin-resistant enterococci (VRE) poses a formidable challenge to public health due to its inherent resistance to multiple antibiotics coupled with the ability to transfer genetic determinants to dangerous pathogens like Methicillin-resistant Staphylococcus aureus (MRSA). The purpose of this st...

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Published in:	Saudi journal of biological sciences Vol. 28; no. 5; pp. 2895 - 2899
Main Authors:	Kankalil George, Siju, Suseela, M.R., El Safi, Saleh, Ali Elnagi, Elmoeiz, Al-Naam, Yaser A, Adlan Mohammed Adam, Ahmed, Mary Jacob, Ashly, Al-Maqati, Thekra, Kumar KS, Harish
Format:	Journal Article
Language:	English
Published:	Riyadh, Saudi Arabia Elsevier B.V 01-05-2021 Saudi Biological Society Elsevier
Subjects:	Antimicrobial resistance Multiplex PCR Original Vancomycin-resistant enterococci Multiplex PCR Antimicrobial resistance Vancomycin-resistant enterococci
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Summary:	Vancomycin-resistant enterococci (VRE) poses a formidable challenge to public health due to its inherent resistance to multiple antibiotics coupled with the ability to transfer genetic determinants to dangerous pathogens like Methicillin-resistant Staphylococcus aureus (MRSA). The purpose of this study was to investigate the incidence of vancomycin resistance in enterococci among clinical isolates at a tertiary care military hospital in the eastern region of Saudi Arabia and to detect van genes using multiplex-PCR. Overall, 246 isolates of enterococci were collected from various clinical specimens. The isolates were identified, and antimicrobial susceptibility testing was done using the Vitek 2 system. Multiplex PCR was performed on the VRE isolates, thus identified to determine the van genes harbored. A total of 15 VRE were identified, of which 14 (93.3%) were Enterococcus faecium, and 1(6.7%) was Enterococcus casseliflavus with intrinsic vanC resistance. Of the 14 vancomycin-resistant Enterococcus faecium, 8 (57.1%) harbored vanB genes, while 6 (42.8%) harbored vanA genes. All the VRE were susceptible to linezolid and tigecycline. Our study detected a low prevalence (6.1%) of VRE among clinical isolates of enterococci and that the vanB gene predominates in such strains. Susceptibility profiles indicated that linezolid and tigecycline are still effective against these multidrug-resistant pathogens. Pus specimens yielded the highest percentage (53.3%) of isolates from which VRE was obtained, and this finding is novel among studies done in Saudi Arabia.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1319-562X 2213-7106
DOI:	10.1016/j.sjbs.2021.02.022