Methylene tetrahydrofolate reductase gene mutation in sickle cell anaemia patients in Lagos, Nigeria

The significant causes of mortality among individuals with sickle cell anaemia (SCA) such as acute chest syndrome and cerebrovascular disease are related to vascular occlusion. Polymorphisms of the methylene tetrahydrofolate reductase (MTHFR) gene in persons with sickle cell anaemia have been sugges...

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Bibliographic Details
Published in:	The Pan African medical journal Vol. 34; no. 213; p. 213
Main Authors:	Adelekan, Oluwaseun Olabisi, Uche, Ebele Ifeyinwa, Balogun, Taiwo Modupe, Osunkalu, Vincent Oluseye, Akinbami, Akinsegun Abduljaleel, Ismail, Kamal Ayobami, Badiru, Mulikat Adesola, Dosunmu, Adedoyin Owolabi, Kamson, Omolara Risqat
Format:	Journal Article
Language:	English
Published:	Uganda African Field Epidemiology Network 2019 The African Field Epidemiology Network The Pan African Medical Journal
Subjects:	a1298c Acute Chest Syndrome - epidemiology Acute Chest Syndrome - mortality Adult Adults Alleles Anemia, Sickle Cell - epidemiology Anemia, Sickle Cell - genetics Anemia, Sickle Cell - mortality Arterial Occlusive Diseases - complications c677t Case-Control Studies Cellulose acetate Cerebrovascular Disorders - epidemiology Cerebrovascular Disorders - mortality Cross-Sectional Studies Deoxyribonucleic acid DNA DNA methylation Enzymes Female Genetic Predisposition to Disease Genotype & phenotype Health care Hematology Hemoglobin Hemoglobin, Sickle - genetics Homocysteine Humans Male Medical research methylene tetrahydrofolate reductase gene mutation Methylenetetrahydrofolate Reductase (NADPH2) - genetics Methylenetetrahydrofolate Reductase (NADPH2) - metabolism Middle Aged Mortality Mutation Nigeria - epidemiology Phenotype Polymorphism, Single Nucleotide - genetics Risk Factors sickle cell anaemia Sickle cell anemia Vascular Diseases - pathology Vitamin B Nigeria Africa C677T Sickle cell anaemia methylene tetrahydrofolate reductase gene mutation A1298C
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Summary:	The significant causes of mortality among individuals with sickle cell anaemia (SCA) such as acute chest syndrome and cerebrovascular disease are related to vascular occlusion. Polymorphisms of the methylene tetrahydrofolate reductase (MTHFR) gene in persons with sickle cell anaemia have been suggested as a potential risk for vaso-occlusive events, with the C677T and A1298C polymorphisms being the commonest. This study therefore aimed to establish the pattern of MTHFR C677T and A1298C gene mutations among adults with HbSS phenotype attending the Haematology Clinic in Lagos State University Teaching Hospital Lagos, Nigeria. A cross-sectional study was done among SCA patients attending the Haematology Clinic of the Lagos State University Teaching Hospital (LASUTH), using age and sex matched HbAA controls. DNA extraction and gene analysis were done. The selective amplification of a particular segment of the DNA by polymerase chain reaction (PCR) was done and subsequent digestion of the amplified MTHFR gene into its various fragments. The overall prevalence of the C677T mutation among participants was 19.3% (37 of 192), while the prevalence of A1298C was 15% (29 of 192). The prevalence of MTHFR C677T was higher than A1298C mutations among sickle cell anaemia subjects.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1937-8688 1937-8688
DOI:	10.11604/pamj.2019.34.213.19524