Comparison of the Effects of GMCSF-Containing and Traditional Culture Media on Embryo Development and Pregnancy Success Rates

The use of granulocyte macrophage colony-stimulating factor (GM-CSF)-containing medium, which is a commercial medium that is used for cultivation of embryos in in vitro fertilization (IVF) treatments, has been suggested to increase the efficiency of this procedure in patients with previous multiple...

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Published in:	Revista Brasileira de ginecologia e obstetrícia Vol. 44; no. 11; pp. 1047 - 1051
Main Authors:	Adanacıoglu, Fatih, Çetin, Çağlar, Tokat, Gozde, Adanacıoglu, Durdane, Karasu, Ayse Filiz Gokmen, Çetin, Mehmet Turan
Format:	Journal Article
Language:	English
Published:	Brazil Thieme Revinter Publicações Ltda 01-11-2022 Federação Brasileira das Sociedades de Ginecologia e Obstetrícia
Subjects:	Case-Control Studies Culture Media - pharmacology embryo culture embryo transfer Embryonic Development Female Fertilization in Vitro granulocyte macrophage colony-stimulating factor Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology Humans in vitro fertilization OBSTETRICS & GYNECOLOGY Original Pregnancy Pregnancy Rate Retrospective Studies granulocyte macrophage colony-stimulating factor in vitro fertilization embryo transfer embryo culture
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Summary:	The use of granulocyte macrophage colony-stimulating factor (GM-CSF)-containing medium, which is a commercial medium that is used for cultivation of embryos in in vitro fertilization (IVF) treatments, has been suggested to increase the efficiency of this procedure in patients with previous multiple unsuccessful attempts. In this retrospective study, we analyzed GM-CSF-containing embryo culture media compared with traditional culture media in terms of development of embryos, pregnancy, and ongoing pregnancy success and live birth rates. This is a prospective case control study conducted in a single center. A total of 131 unexplained infertility patients were included in the study. A cohort of 69 patients whose embryos were cultured in GM-CSF-containing medium and a control group of 62 age-matched patients whose embryos were cultured in conventional Sage One Step medium were included in the study. The major study outcomes were achievement of pregnancy and ongoing pregnancy rate at 12 weeks of gestation. The pregnancy and ongoing pregnancy rates of the patients whose embryos were cultured in GM-CSF-containing medium were 39.13% and 36.23%, respectively. These were higher than the rates of the control group, which were 30.65% and 29.03%, respectively, although this difference was not statistically significant. In addition, the 5 -day embryo transfer percentage in the GM-CSF group was higher than in the control group (34.78% versus 27.4%). The main findings of our study were that there was no difference between the GM-CSF-enhanced medium and the control group in terms of our major study outcomes. However, blastomere inequality rate and embryo fragmentation rates were lower in the GM-CSF group.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0100-7203 1806-9339 1806-9339
DOI:	10.1055/s-0042-1759630