Interaction of the apolipoprotein E receptors low density lipoprotein receptor-related protein and sorLA/LR11

Abstract In this study, we examined protein–protein interactions between two neuronal receptors, low density lipoprotein receptor-related protein (LRP) and sorLA/LR11, and found that these receptors interact, as indicated by three independent lines of evidence: co-immunoprecipitation experiments on...

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Published in:	Neuroscience Vol. 158; no. 4; pp. 1460 - 1468
Main Authors:	Spoelgen, R, Adams, K.W, Koker, M, Thomas, A.V, Andersen, O.M, Hallett, P.J, Bercury, K.K, Joyner, D.F, Deng, M, Stoothoff, W.H, Strickland, D.K, Willnow, T.E, Hyman, B.T
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Ltd 18-02-2009 Elsevier
Subjects:	Animals Binding Sites Biological and medical sciences Cells, Cultured Embryo, Mammalian Fundamental and applied biological sciences. Psychology Green Fluorescent Proteins - genetics Humans Immunoprecipitation - methods LDL receptor LDL-Receptor Related Proteins - genetics LDL-Receptor Related Proteins - metabolism LR11 LRP Mice Microscopy, Fluorescence Neuroblastoma Neurology Neurons - metabolism Protein Binding Protein Interaction Domains and Motifs Protein Interaction Mapping - methods Rats Rats, Sprague-Dawley Receptors, LDL - genetics Receptors, LDL - metabolism sorLA Surface Plasmon Resonance - methods Transfection - methods Vertebrates: nervous system and sense organs APP sorLA LR11 apoE apolipoprotein E receptor-2 platelet-derived growth factor FLIM low density lipoprotein receptor LRP ligand-binding domain LBD mLRP Neuro2a GFP Aβ Tris-buffered saline amyloid precursor protein low density lipoprotein receptor-related protein FBS Vsp10p FRET fluorescence lifetime imaging microscopy NDS Tris-buffered saline containing 0.05% Tween-20 TBS normal donkey serum mini–low density lipoprotein receptor-related protein receptor amyloid-β fluorescence resonance energy transfer TBST low density lipoprotein receptor-related protein light chain ApoER2 green fluorescent protein LDL receptor fetal bovine serum LDL-r PDGF vacuolar protein sorting 10 protein LC apolipoprotein E N2a Apolipoprotein E Lipoprotein Density Protein Biological receptor
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Summary:	Abstract In this study, we examined protein–protein interactions between two neuronal receptors, low density lipoprotein receptor-related protein (LRP) and sorLA/LR11, and found that these receptors interact, as indicated by three independent lines of evidence: co-immunoprecipitation experiments on mouse brain extracts and mouse neuronal cells, surface plasmon resonance analysis with purified human LRP and sorLA, and fluorescence lifetime imaging microscopy (FLIM) on rat primary cortical neurons. Immunocytochemistry experiments revealed widespread co-localization of LRP and sorLA within perinuclear compartments of rat primary neurons, while FLIM analysis showed that LRP-sorLA interactions take place within a subset of these compartments.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0306-4522 1873-7544
DOI:	10.1016/j.neuroscience.2008.10.061