Search Results - "Adam Thean Chor Leow"
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Biosynthesis of agar in red seaweeds: A review
Published in Carbohydrate polymers (15-05-2017)“…•Agar is a sulfated galactan synthesized in the cell wall matrix of red seaweeds.•The gaps in current knowledges on agar biosynthesis in red seaweeds are…”
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Factors affecting yield and gelling properties of agar
Published in Journal of applied phycology (01-06-2017)“…Agar, a gelatinous polysaccharide in the cell wall of many red algal species, is widely used as a gelling, thickening and stabilizing agent. The commercial…”
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Single Residue Substitution at N -Terminal Affects Temperature Stability and Activity of L2 Lipase
Published in Molecules (Basel, Switzerland) (28-07-2020)“…Rational design is widely employed in protein engineering to tailor wild-type enzymes for industrial applications. The typical target region for mutation is a…”
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Ion-Pair Interaction and Hydrogen Bonds as Main Features of Protein Thermostability in Mutated T1 Recombinant Lipase Originating from Geobacillus zalihae
Published in Molecules (Basel, Switzerland) (28-07-2020)“…A comparative structure analysis between space- and an Earth-grown T1 recombinant lipase from had shown changes in the formation of hydrogen bonds and ion-pair…”
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Expression, Characterisation and Homology Modelling of a Novel Hormone-Sensitive Lipase (HSL)-Like Esterase from Glaciozyma antarctica
Published in Catalysts (01-01-2020)“…Microorganisms, especially those that survive in extremely cold places such as Antarctica, have gained research attention since they produce a unique feature…”
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Danger lurking in the "unknowns": structure-to-function studies of hypothetical protein Bleg1_2437 from Bacillus lehensis G1 alkaliphile revealed an evolutionary divergent B3 metallo-beta-lactamase
Published in Journal of biochemistry (Tokyo) (01-02-2017)“…The effectiveness of β-lactam antibiotics as chemotherapeutic agents to treat bacterial infections is gradually threatened with the emergence of antibiotic…”
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Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3
Published in PeerJ (San Francisco, CA) (20-10-2016)“…A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic strain AMS3. The recombinant lipase AMS3 was purified by single-step…”
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Enhancement of a protocol purifying T1 lipase through molecular approach
Published in PeerJ (San Francisco, CA) (16-11-2018)“…T1 Lipase is a thermostable secretary protein of strain previously expressed in a prokaryotic system and purified using three-step purification: affinity 1,…”
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Crystallographic Analysis of Ground and Space Thermostable T1 Lipase Crystal Obtained via Counter Diffusion Method Approach
Published in BioMed research international (01-01-2014)“…Three-dimensional structure of thermostable lipase is much sought after nowadays as it is important for industrial application mainly found in the food,…”
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The biology and the importance of Photobacterium species
Published in Applied microbiology and biotechnology (01-06-2017)“…Photobacterium species are Gram-negative coccobacilli which are distributed in marine habitats worldwide. Some species are unique because of their capability…”
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Capillary-seeding crystallization and preliminary crystallographic analysis of a solvent-tolerant elastase from Pseudomonas aeruginosa strain K
Published in International journal of molecular sciences (28-08-2013)“…Seeding is a versatile method for optimizing crystal growth. Coupling this technique with capillary counter diffusion crystallization enhances the size and…”
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Polyunsaturated fatty acids in marine bacteria and strategies to enhance their production
Published in Applied microbiology and biotechnology (01-07-2018)“…Polyunsaturated fatty acids (PUFAs) play an important role in human diet. Despite the wide-ranging importance and benefits from heart health to brain…”
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Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues
Published in International journal of molecular sciences (06-12-2022)“…Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth's biosphere. One of the…”
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Structure elucidation and docking analysis of 5M mutant of T1 lipase Geobacillus zalihae
Published in PloS one (01-06-2021)“…5M mutant lipase was derived through cumulative mutagenesis of amino acid residues (D43E/T118N/E226D/E250L/N304E) of T1 lipase from Geobacillus zalihae. A…”
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Effective refolding of a cysteine rich glycoside hydrolase family 19 recombinant chitinase from Streptomyces griseus by reverse dilution and affinity chromatography
Published in PloS one (22-10-2020)“…Conventional refolding methods are associated with low yields due to misfolding and high aggregation rates or very dilute proteins. In this study, we describe…”
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Clinical and Preclinical Studies of Fermented Foods and Their Effects on Alzheimer's Disease
Published in Antioxidants (29-04-2022)“…The focus on managing Alzheimer's disease (AD) is shifting towards prevention through lifestyle modification instead of treatments since the currently…”
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The Role of Surface Exposed Lysine in Conformational Stability and Functional Properties of Lipase from Staphylococcus Family
Published in Molecules (Basel, Switzerland) (25-08-2020)“…Surface charge residues have been recognized as one of the stability determinants in protein. In this study, we sought to compare and analyse the stability and…”
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Enhancement in T1 lipase purification recovery using the novel construct pGEX4T1/His-T1
Published in Preparative biochemistry & biotechnology (01-04-2024)“…The Geobacillus zalihae strain T1 produces a thermostable T1 lipase that could be used for industrial purposes. Previously, the GST-T1 lipase was purified…”
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Altering the Regioselectivity of T1 Lipase from Geobacillus zalihae toward sn-3 Acylglycerol Using a Rational Design Approach
Published in Catalysts (01-02-2023)“…The regioselectivity characteristic of lipases facilitate a wide range of novel molecule unit constructions and fat modifications. Lipases can be categorized…”
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The Role of Solvent-Accessible Leu-208 of Cold-Active Pseudomonas fluorescens Strain AMS8 Lipase in Interfacial Activation, Substrate Accessibility and Low-Molecular Weight Esterification in the Presence of Toluene
Published in Molecules (Basel, Switzerland) (12-08-2017)“…The alkaline cold-active lipase from AMS8 undergoes major structural changes when reacted with hydrophobic organic solvents. In toluene, the AMS8 lipase…”
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