Marked Increase in Number of Dendritic Cells in Autoimmune‐Prone (NZW × BXSB)F1 Mice with Age

Here, we report that the number of CD11c+CD3− B220− cells increases in autoimmune‐prone male (NZW × BXSB)F1 (W/BF1) mice with age. The CD11c+CD3−B220− cells from W/BF1 mice show a typical stellate shape and induce the proliferation of T cells. In the CD11c+CD3−B220− cells from W/BF1 mice, CD11b (Mac...

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Published in:	Stem cells (Dayton, Ohio) Vol. 20; no. 1; pp. 61 - 72
Main Authors:	Adachi, Yashusi, Taketani, Shigeru, Toki, Junko, Ikebukuro, Kazuya, Sugiura, Kikuya, Oyaizu, Haruki, Yasumizu, Ryoji, Tomita, Minoru, Kaneda, Hiroyuki, Amoh, Yasuo, Ito, Tomoki, Okigaki, Mitsuhiko, Inaba, Muneo, Ikehara, Susumu
Format:	Journal Article
Language:	English
Published:	Bristol John Wiley & Sons, Ltd 01-01-2002
Subjects:	(NZW × BXSB)F1 mouse Animals Autoimmune disease Bone Marrow - metabolism CD11 Antigens - biosynthesis CD4-Positive T-Lymphocytes - metabolism CD8 Antigens - biosynthesis Cell Division Cell Line Cell Membrane - metabolism Crosses, Genetic Dendritic cell Dendritic Cells - cytology Dendritic Cells - metabolism Endocytosis fas Receptor - biosynthesis Flow Cytometry Flt‐3 ligand fms-Like Tyrosine Kinase 3 Integrin alphaXbeta2 - biosynthesis Leukocytes, Mononuclear - metabolism Lymphocytes - metabolism Membrane Proteins - biosynthesis Mice Phagocytosis Proto-Oncogene Proteins - biosynthesis Proto-Oncogene Proteins c-kit - biosynthesis Receptor Protein-Tyrosine Kinases - biosynthesis Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Thrombocytopenia - metabolism Time Factors Up-Regulation
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Summary:	Here, we report that the number of CD11c+CD3− B220− cells increases in autoimmune‐prone male (NZW × BXSB)F1 (W/BF1) mice with age. The CD11c+CD3−B220− cells from W/BF1 mice show a typical stellate shape and induce the proliferation of T cells. In the CD11c+CD3−B220− cells from W/BF1 mice, CD11b (Mac‐1α), NK 1.1, and CD95 (Fas) are upregulated in comparison with normal mice, while the expression of CD8α, CD117 (c‐kit), CD135 (Flk‐2/Flt‐3), and Sca‐1 decreases. There is a significant increase in Flt‐3L (FL) mRNA in the bone marrow of W/BF1 mice with age. Moreover, activated hemopoietic cells express high levels of FL. The injection of CD11c+CD3−B220− cells from old W/BF1 mice to young W/BF1 mice transiently induces autoimmune disease (thrombocytopenia). These results suggest that hyperproduction of FL from activated hemopoietic cells induces a dramatic increase in the number of dendritic cells in aged W/BF1 mice, followed by the acceleration of autoimmunity.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1066-5099 1549-4918
DOI:	10.1634/stemcells.20-1-61