Search Results - "Ackers, G K"

Coupled Energetics of λ cro Repressor Self-Assembly and Site-Specific DNA Operator Binding I:  Analysis of cro Dimerization from Nanomolar to Micromolar Concentrations by Darling, Paul J, Holt, Jo M, Ackers, Gary K

“…The cro repressor from bacteriophage λ is an important and classical transcription regulatory protein that binds DNA operator sites as a dimer. Therefore, a…”
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Molecular Code for Cooperativity in Hemoglobin by Ackers, Gary K., Doyle, Michael L., Myers, David, Daugherty, Margaret A.

“…Although tetrameric hemoglobin has been studied extensively as a prototype for understanding mechanisms of allosteric regulation, the functional and structural…”
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Footprint'' Titrations Yield Valid Thermodynamic Isotherms by Brenowitz, Michael, Senear, Donald F., Shea, Madeline A., Ackers, Gary K.

“…A central issue in gene regulation is the mechanism, and biological function, of the cooperative binding of regulatory protein ligands to specific sites on…”
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The OR control system of bacteriophage lambda. A physical-chemical model for gene regulation by Shea, M A, Ackers, G K

“…A quantitative model has been developed for processes in the bacteriophage lambda that control the switchover from lysogenic to lytic modes of growth. These…”
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Quantitative Model for Gene Regulation by λ Phage Repressor by Ackers, Gary K., Johnson, Alexander D., Shea, Madeline A.

“…A statistical thermodynamic model has been developed to account for the cooperative interactions of the bacteriophage λ repressor with the λ right operator…”
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Experimental Resolution of Cooperative Free Energies for the Ten Ligation States of Human Hemoglobin by Smith, Francine R., Ackers, Gary K.

“…Tetrameric human hemoglobin can assume ten molecular forms that differ in the number and configuration of ligands bound at the four heme sites. For each of…”
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Site-specific enthalpic regulation of DNA transcription at bacteriophage .lambda. OR by Koblan, Kenneth S, Ackers, Gary K

“…Binding of cI repressor to DNA fragments containing the three specific binding sites of the right operator (OR) of bacteriophage lambda was studied in vitro…”
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Hydrogen Exchange Measurement of the Free Energy of Structural and Allosteric Change in Hemoglobin by Englander, S. Walter, Englander, Joan J., McKinnie, Russell E., Ackers, Gary K., Turner, George J., Westrick, Judy A., Gill, Stanley J.

“…The inability to localize and measure the free energy of protein structure and structure change severely limits protein structure-function investigations. The…”
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Identification of the Intermediate Allosteric Species in Human Hemoglobin Reveals a Molecular Code for Cooperative Switching by Daugherty, Margaret A., Shea, Madeline A., Johnson, Jeremy A., LiCata, Vince J., Turner, George J., Ackers, Gary K.

“…The 10 ligation species of human cyanomethemoglobin were previously found to distribute into three discrete cooperative free energy levels according to a…”
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Mutagenic dissection of hemoglobin cooperativity: effects of amino acid alteration on subunit assembly of oxy and deoxy tetramers by Turner, G J, Galacteros, F, Doyle, M L, Hedlund, B, Pettigrew, D W, Turner, B W, Smith, F R, Moo-Penn, W, Rucknagel, D L, Ackers, G K

“…Free energies of oxygen-linked subunit assembly and cooperative interaction have been determined for 34 molecular species of human hemoglobin, which differ by…”
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Energetics of subunit dimerization in bacteriophage .lambda. cI repressor: linkage to protons, temperature, and KCl by Koblan, Kenneth S, Ackers, Gary K

“…A common feature of gene regulatory systems is the linkage between reversible protein oligomerization and DNA binding. Experimental dissection using…”
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Cooperative protein-DNA interactions : effects of KCl on λ cI binding to OR by KOBLAN, K. S, ACKERS, G. K

“…The effects of monovalent salt activity on the site-specific and cooperative interactions of cI repressor with its three operator sites OR were studied by…”
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Effects of Site-Specific Amino Acid Modification on Protein Interactions and Biological Function by Ackers, G K, Smith, F R

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Isolation of .lambda. repressor mutants with defects in cooperative operator binding by Beckett, Dorothy, Burz, David S, Ackers, Gary K, Sauer, Robert T

“…A hybrid operator-promoter region was designed to aid in a screen for cooperativity mutants of the lambda repressor. In this system, lambda repressor mutants…”
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Heterometallic Hybrids of Homometallic Human Hemoglobins by Huang, Yingwen, Yonetani, Takashi, Tsuneshige, Antonio, Hoffman, Brain M., Ackers, Gary K.

“…Hybridization experiments between normal Hb tetramers (Fe2+Hb) and those with four metal-substituted hemes (i.e., replacement of Fe2+ by Co2+, Mg2+, Mn2+,…”
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Functional properties of human hemoglobins synthesized from recombinant mutant .beta.-globins by Doyle, Michael L, Lew, George, De Young, Alice, Kwiatkowski, Laura, Wierzba, Anita, Noble, Robert W, Ackers, Gary K

“…The previous and following articles in this issue describe the recombinant synthesis of three mutant beta-globins (beta 1 Val---Ala, beta 1 Val---Met, and the…”
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Energetics of cooperative protein-DNA interactions: comparison between quantitative deoxyribonuclease footprint titration and filter binding by Senear, Donald F, Brenowitz, Michael, Shea, Madeline A, Ackers, Gary K

“…Using the binding of cI repressor protein to the lambda right and left operators as a model system, we have analyzed the two common experimental techniques for…”
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Proton-linked contributions to site-specific interactions of .lambda. cI repressor and OR by Senear, Donald F, Ackers, Gary K

“…The effects of proton activity on the site-specific interactions of cI repressors with operator sites OR were studied by using DNase I footprint titration…”
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Mutual effects of protons, NaCl, and oxygen on the dimer-tetramer assembly of human hemoglobin. The dimer Bohr effect by Chu, A H, Ackers, G K

“…The dimer-tetramer equilibrium constants of human oxyhemoglobin (4K2) and deoxyhemoglobin (0K2) have been determined at 21.5 degrees C as a function of pH and…”
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Quantitative study of protein association at picomolar concentrations: the lambda phage cl repressor by Beckett, D, Koblan, K S, Ackers, G K

“…A method has been developed for radiolabeling the lambda cl repressor to a specific activity sufficiently high to permit accurate quantitation of the protein…”
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