Search Results - "Achyuthan, K E"

Identification of a guanosine triphosphate-binding site on guinea pig liver transglutaminase. Role of GTP and calcium ions in modulating activity by Achyuthan, K E, Greenberg, C S

“…Guanosine 5'-triphosphate (GTP) was found to inhibit guinea pig liver transglutaminase activity as measured by [3H]putrescine incorporation into casein. GDP…”
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Rapid diagnostics: the detection of neuraminidase activity as a technology for high-specificity targets by Shimasaki, C. D., Achyuthan, K. E., Hansjergen, J. A., Appleman, J. R.

“…The accurate detection of influenza by clinical symptoms is challenging since multiple pathogenic viruses and bacteria mimic similar symptoms in a patient…”
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A microtiter plate transglutaminase assay utilizing 5-(biotinamido)pentylamine as substrate by Slaughter, T F, Achyuthan, K E, Lai, T S, Greenberg, C S

“…Transglutaminases belong to an important family of enzymes involved in hemostasis, skin formation, and wound healing. We describe a technique for the…”
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Comparative enzymology, biochemistry and pathophysiology of human exo-α-sialidases (neuraminidases) by Achyuthan, Komandoor E., Achyuthan, Ann M.

“…This review summarizes the current research on human exo-α-sialidase (sialidase, neuraminidase). Where appropriate, the properties of viral, bacterial, and…”
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The binding sites on fibrin(ogen) for guinea pig liver transglutaminase are similar to those of blood coagulation factor XIII. Characterization of the binding of liver transglutaminase to fibrin by Achyuthan, K E, Mary, A, Greenberg, C S

“…The present study represents detailed investigations into the nature of interactions between an intracellular “tissue” transglutaminase and a plasma protein,…”
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Enhanced chemiluminescent assay for transglutaminases by Achyuthan, K E

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Factor XIIIa-derived peptides inhibit transglutaminase activity. Localization of substrate recognition sites by ACHYUTHAN, K. E, SLAUGHTER, T. F, SANTIAGO, M. A, ENGHILD, J. J, GREENBERG, C. S

“…Factor XIIIa is a transglutaminase that catalyzes intermolecular gamma-glutamyl-epsilon-lysyl bonds between fibrin and other proteins involved in hemostasis…”
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Factor XIIIa formation promoted by complexing of α-thrombin, fibrin, and plasma factor XIII by GREENBERG, C. S, ACHYUTHAN, K. E, FENTON, J. W. II

“…Fibrin polymers (des A,B fibrinogen) reduced the concentration of alpha-thrombin required for 50% activation of plasma factor XIII (a2b2 tetramer) by…”
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Vitronectin is a substrate for transglutaminases by Sane, D C, Moser, T L, Pippen, A M, Parker, C J, Achyuthan, K E, Greenberg, C S

“…Vitronectin (VN) was found to be a substrate for both plasma transglutaminase (Factor XIIIa) and guinea pig liver transglutaminase (TG). Incorporation of…”
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Carboxyl-terminal truncation of recombinant factor XIII A-chains. Characterization of minimum structural requirement for transglutaminase activity by Lai, T S, Achyuthan, K E, Santiago, M A, Greenberg, G S

“…A series of truncation mutants lacking 218, 229, 250, and 269 amino acid residues from the carboxyl terminus of blood coagulation factor XIII A-chains (FXIII…”
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Immunochemical analyses of human plasma fibronectin-cytosolic transglutaminase interactions by Achyuthan, Komandoor E., Jeff Goodell, R., Kennedye, James R., Lee, Kyung N., Henley, Anna, Stiefer, John R., Birckbichler, Paul J.

“…Fibronectin is a glycoprotein involved in cell adhesion, tissue organization and wound healing. Transglutaminase binding and covalent cross-linking of…”
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b-chains prevent the proteolytic inactivation of the a-chains of plasma factor XIII by Mary, A, Achyuthan, K E, Greenberg, C S

“…While the transglutaminase activity is associated exclusively with the thrombin-cleaved a chains of plasma Factor XIII, there is little information regarding…”
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The Transglutaminase in Vascular Cells and Tissues Could Provide an Alternate Pathway for Fibrin Stabilization by Greenberg, Charles S., Achyuthan, Komandoor E., Borowitz, Michael J., Shuman, Marc A.

“…A thrombin-independent transglutaminase (TG) has been identified in vascular cells and tissues from human, rabbit, rat, porcine, and bovine sources. The…”
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A microtiter plate assay for factor XIII A-chain-fibrin interactions by Achyuthan, K E, Santiago, M A, Greenberg, C S

“…Factor XIII A-chain-fibrin interactions regulate factor XIIIa formation and fibrin cross-linking. A microtiter plate assay was developed for studying these…”
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Isolation of a fibrin-binding fragment from blood coagulation factor XIII capable of cross-linking fibrin(ogen) by Greenberg, C S, Enghild, J J, Mary, A, Dobson, J V, Achyuthan, K E

“…Purified platelet Factor XIII was radioiodinated and then partially degraded by thrombin or trypsin, and a fibrin-binding fragment was identified by…”
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Tb(III)-ion-binding-induced conformational changes in platelet factor XIII by Achyuthan, K E, Mary, A, Greenberg, C S

“…Ca(II) ions are crucial during proteolytic conversion of Factor XIII zymogen into the active enzyme Factor XIIIa. Factor XIII proteolyzed by thrombin or…”
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Factor XIII binds to the A alpha- and B beta- chains in the D-domain of fibrinogen: an immunoblotting study by Mary, A, Achyuthan, K E, Greenberg, C S

“…The binding sites in fibrinogen for Factor XIII were localized using an immunoblotting technique. Platelet Factor XIII bound to fibrinogen and to plasmin…”
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The binding of divalent metal ions to platelet factor XIII modulates its proteolysis by trypsin and thrombin by Mary, A, Achyuthan, K E, Greenberg, C S

“…We investigated the effect of divalent metal ions on the proteolytic cleavage and activation of platelet Factor XIII by thrombin and trypsin. In the absence of…”
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Characterization of the reciprocal binding sites on human alpha-thrombin and factor XIII A-chain by Achyuthan, K E

“…Solution- and solid-phase techniques were used to probe Factor XIII A-chain-alpha-thrombin interactions. Alpha-thrombin activated Factor XIII more efficiently…”
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Characterization of the fibrin polymer structure that accelerates thrombin cleavage of plasma factor XIII by Greenberg, C S, Achyuthan, K E, Rajagopalan, S, Pizzo, S V

“…The effect of plasmin-derived fibrin(ogen) degradation products on alpha-thrombin cleavage of plasma Factor XIII was studied to identify the fibrin polymer…”
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