Relationships between cagA, vacA, and iceA genotypes of Helicobacter pylori and DNA damage in the gastric mucosa

Helicobacter pylori (H. pylori) is believed to predispose carriers to gastric cancer by inducing chronic inflammation. The inflammatory processes may result in the generation of reactive oxygen and nitrogen species that damage DNA. In this study, we investigated the relationships between DNA damage...

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Published in:	Environmental and molecular mutagenesis Vol. 44; no. 2; pp. 91 - 98
Main Authors:	Ladeira, Marcelo S.P., Rodrigues, Maria A.M., Salvadori, Daisy M.F., Neto, Pedro Padulla, Achilles, Pedro, Lerco, Mauro M., Rodrigues, Paulo A., Gonçalves Jr, Írio, Queiroz, Dulciene M.M., Freire-Maia, Dértia V.
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 2004 Wiley-Liss
Subjects:	Adult Aged Antigens, Bacterial - genetics Bacterial Proteins - genetics Base Sequence Biological and medical sciences Comet Assay DNA Damage DNA Primers Female Fundamental and applied biological sciences. Psychology Gastric Mucosa - metabolism Genetics of eukaryotes. Biological and molecular evolution Genotype genotypes Helicobacter pylori Helicobacter pylori - genetics Humans Male Medical sciences Middle Aged Toxicology Spirillales Mutagenicity testing Mucosa DNA damage Spirillaceae genotypes Genotype Comet assay Toxicology Helicobacter pylori DNA Bacteria Genetics Lesion
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Summary:	Helicobacter pylori (H. pylori) is believed to predispose carriers to gastric cancer by inducing chronic inflammation. The inflammatory processes may result in the generation of reactive oxygen and nitrogen species that damage DNA. In this study, we investigated the relationships between DNA damage in the gastric mucosa and cagA, vacA, and iceA genotypes of H. pylori. The study was conducted with biopsies from the gastric antrum and corpus of 98 H. pylori‐infected and 26 uninfected control patients. H. pylori genotypes were determined by PCR and DNA damage was measured in gastric mucosal cells by the Comet assay (single cell gel electrophoresis). All patients were nonsmokers, not abusing alcohol, and not using prescription or recreational drugs. Levels of DNA damage were significantly higher (P < 0.0001) in the H. pylori‐infected patients than in uninfected patients. In comparison with the level of DNA damage in the uninfected controls, the extent of DNA damage in both the antrum (OR = 8.45; 95% CI = 2.33–37.72) and the corpus (OR = 6.55; 95% CI = 2.52–17.72) was related to infection by cagA+/vacAs1m1 and iceA1 strains. The results indicate that the genotype of H. pylori is related to the amount of DNA damage in the gastric mucosa. These genotypes could serve as biomarkers for the risk of extensive DNA damage and possibly gastric cancer. Environ. Mol. Mutagen. 44:91–98, 2004. © 2004 Wiley‐Liss, Inc.
Bibliography:	Fundação de Amparo à Pesquisa do Estado de São Paulo - No. 97/05026-3; No. 03/09217-0; No. 97/11839-7; No. 03/05043-5 istex:6CF4B5BD7EF373EE6BBC61023D9CC5AD29B8C823 ArticleID:EM20045 ark:/67375/WNG-H23X0308-N ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0893-6692 1098-2280
DOI:	10.1002/em.20045