No more time to stay 'single' in the detection of Anisakis pegreffii, A. simplex (s. s.) and hybridization events between them: a multi-marker nuclear genotyping approach

A multi-marker nuclear genotyping approach was performed on larval and adult specimens of Anisakis spp. (N = 689) collected from fish and cetaceans in allopatric and sympatric areas of the two species Anisakis pegreffii and Anisakis simplex (s. s.), in order to: (1) identify specimens belonging to t...

Full description

Saved in:

Bibliographic Details
Published in:	Parasitology Vol. 143; no. 8; p. 998
Main Authors:	Mattiucci, S, Acerra, V, Paoletti, M, Cipriani, P, Levsen, A, Webb, S C, Canestrelli, D, Nascetti, G
Format:	Journal Article
Language:	English
Published:	England 01-07-2016
Subjects:	Animals Anisakiasis - parasitology Anisakiasis - veterinary Anisakis - genetics Anisakis - isolation & purification Cell Nucleus - genetics DNA, Helminth - genetics DNA, Ribosomal Spacer - genetics Female Fish Diseases - parasitology Fishes Genetic Markers - genetics Genotype Geography Hybridization, Genetic Larva Male Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length Species Specificity A. simplex (s. s.) multi-marker genotyping approach Anisakis pegreffii contemporary hybridization elongation factor 1 alpha1 nDNA (EF1 α-1 nDNA region)
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A multi-marker nuclear genotyping approach was performed on larval and adult specimens of Anisakis spp. (N = 689) collected from fish and cetaceans in allopatric and sympatric areas of the two species Anisakis pegreffii and Anisakis simplex (s. s.), in order to: (1) identify specimens belonging to the parental taxa by using nuclear markers (allozymes loci) and sequence analysis of a new diagnostic nuclear DNA locus (i.e. partial sequence of the EF1 α-1 nDNA region) and (2) recognize hybrid categories. According to the Bayesian clustering algorithms, based on those markers, most of the individuals (N = 678) were identified as the parental species [i.e. A. pegreffii or A. simplex (s. s.)], whereas a smaller portion (N = 11) were recognized as F1 hybrids. Discordant results were obtained when using the polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) of the internal transcribed spacer (ITS) ribosomal DNA (rDNA) on the same specimens, which indicated the occurrence of a large number of 'hybrids' both in sympatry and allopatry. These findings raise the question of possible misidentification of specimens belonging to the two parental Anisakis and their hybrid categories derived from the application of that single marker (i.e. PCR-RFLPs analysis of the ITS of rDNA). Finally, Bayesian clustering, using allozymes and EF1 α-1 nDNA markers, has demonstrated that hybridization between A. pegreffii and A. simplex (s. s.) is a contemporary phenomenon in sympatric areas, while no introgressive hybridization takes place between the two species.
ISSN:	1469-8161
DOI:	10.1017/S0031182016000330