Significant antibacterial activity and synergistic effects of camel lactoferrin with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA)

Significant antibacterial activity and synergistic effects of camel lactoferrin with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA)

Methicillin-resistant Staphylococcus aureus (MRSA) causes major healthcare problems in many countries, as it is present as several hospital- and community-associated strains. Hospital-associated MRSA is one of the most prevalent nosocomial pathogens throughout the world and infections caused by comm...

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Bibliographic Details
Published in:Research in microbiology Vol. 167; no. 6; pp. 480 - 491
Main Authors: Redwan, Elrashdy M., El-Baky, Nawal Abd, Al-Hejin, Ahmed M., Baeshen, Mohammed N., Almehdar, Hussein A., Elsaway, Abdulrahman, Gomaa, Abu-Bakr M., Al-Masaudi, Saad Berki, Al-Fassi, Fahad A., AbuZeid, Isam Eldin, Uversky, Vladimir N.
Format: Journal Article
Language:English
Published: France Elsevier Masson SAS 01-07-2016
Subjects:
Animals
Anti-Infective Agents - pharmacology
Antibiotics
Camelus
Drug Synergism
Humans
Lactoferrin
Lactoferrin - pharmacology
Methicillin-Resistant Staphylococcus aureus - drug effects
Microbial Sensitivity Tests
MRSA
Staphylococcus aureus
Synergistic action
Synergistic action
Lactoferrin
Antibiotics
MRSA
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Summary:Methicillin-resistant Staphylococcus aureus (MRSA) causes major healthcare problems in many countries, as it is present as several hospital- and community-associated strains. Hospital-associated MRSA is one of the most prevalent nosocomial pathogens throughout the world and infections caused by community-acquired MRSA are rising. This emphasizes the need for new and efficient anti-MRSA agents. We evaluated the antibacterial effects of camel lactoferrin (cLf) and human lactoferrin (hLf) alone and in combination with several antibiotics against MRSA. Antimicrobials were tested against MRSA and an S. aureus control strain by the agar disc diffusion method. The minimum inhibitory concentration (MIC) was determined for antimicrobials by the broth microdilution method. Synergy between cLf or hLf and antibiotics was examined by checkerboard and time-kill assays. The agar disc diffusion assay showed that MRSA growth was inhibited by cLf at 0.25–3 mg/ml and hLf at 1–3 mg/ml. cLf demonstrated 3 times higher inhibitory activity against MRSA than hLf in terms of MIC values (250 vs. 750 μg/ml, respectively). Biotinylated cLf was recognized by two membrane proteins of MRSA, 66–67 KDa. Combinations of cLf or hLf and oxacillin or vancomycin at sub-MIC levels enhanced in vitro antibacterial activity against MRSA compared with each agent alone.
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ISSN:0923-2508
1769-7123
DOI:10.1016/j.resmic.2016.04.006