Transplantation of photobiomodulation-preconditioned diabetic stem cells accelerates ischemic wound healing in diabetic rats

Diabetic foot ulcer is the most costly and complex challenge for patients with diabetes. We hereby assessed the effectiveness of different preconditioned adipose-derived mesenchymal stem cells (AD-MSCs) and photobiomodulation protocols on treating an infected ischemic wound in type 1 diabetic rats....

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Bibliographic Details
Published in:	Stem cell research & therapy Vol. 11; no. 1; p. 494
Main Authors:	Ahmadi, Houssein, Amini, Abdollah, Fadaei Fathabady, Fatemeh, Mostafavinia, Atarodsadat, Zare, Fatemeh, Ebrahimpour-Malekshah, Roohollah, Ghalibaf, Mustafa Neshat, Abrisham, Matin, Rezaei, Fatemehalsadat, Albright, Richard, Ghoreishi, Seyed Kamran, Chien, Sufan, Bayat, Mohammad
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 25-11-2020 BioMed Central
Subjects:	Advertising executives Animals Apoptosis Diabetes Diabetes mellitus Diabetes Mellitus, Experimental - therapy Diabetic foot Foot diseases Glucose Humans Hypoxia Inflammation Ischemia Lasers Light therapy Low-Level Light Therapy Medical research Medicine, Experimental Mesenchymal Stem Cell Transplantation Mesenchymal stem cells Mesenchyme Methicillin Rats Rats, Wistar Rodents Skin Staphylococcus aureus infections Staphylococcus infections Stem cell transplantation Stem Cells Surgery Transplantation Type 1 diabetes Wound Healing Adipose-derived mesenchymal stem cells Methicillin-resistant Staphylococcus aureus infection Wound healing Wound closure rate Preconditioning stem cell, photobiomodulation Rats Tensiometric properties Diabetes mellitus Ischemia
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Summary:	Diabetic foot ulcer is the most costly and complex challenge for patients with diabetes. We hereby assessed the effectiveness of different preconditioned adipose-derived mesenchymal stem cells (AD-MSCs) and photobiomodulation protocols on treating an infected ischemic wound in type 1 diabetic rats. There were five groups of rats: (1) control, (2) control AD-MSCs [diabetic AD-MSCs were transplanted (grafted) into the wound bed], (3) AD-MSC + photobiomodulation in vivo (diabetic AD-MSCs were grafted into the wound, followed by in vivo PBM treatment), (4) AD-MSCs + photobiomodulation in vitro, and (5) AD-MSCs + photobiomodulation in vitro + in vivo. Diabetic AD-MSCs preconditioned with photobiomodulation had significantly risen cell function compared to diabetic AD-MSC. Groups 3 and 5 had significantly decreased microbial flora correlated to groups 1 and 2 (all, p = 0.000). Groups 2, 3, 4, and 5 had significantly improved wound closure rate (0.4, 0.4, 0.4, and 0.8, respectively) compared to group 1 (0.2). Groups 2-5 had significantly increased wound strength compared to group 1 (all p = 0.000). In most cases, group 5 had significantly better results than groups 2, 3, and 4. Preconditioning diabetic AD-MSCs with photobiomodulation in vitro plus photobiomodulation in vivo significantly hastened healing in the diabetic rat model of an ischemic infected delayed healing wound.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1757-6512 1757-6512
DOI:	10.1186/s13287-020-01967-2