Molecular and morphometric identification of Trypanosoma (Megatrypanum) minasense in blood samples of marmosets (Callithrix: Callithrichidae) from the city of Rio de Janeiro, Brazil

Molecular and morphometric identification of Trypanosoma (Megatrypanum) minasense in blood samples of marmosets (Callithrix: Callithrichidae) from the city of Rio de Janeiro, Brazil

Callithrix jacchus and C. penicillata marmosets are invasive to the state of Rio de Janeiro, Brazil, threatening the native and vulnerable C. aurita. Both invasive species can be hosts of Trypanosoma cruzi, T. minasense, T. rangeli and T. devei. We aim to investigate the occurrence of trypanosomatid...

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Published in:Parasitology international Vol. 75; p. 101999
Main Authors: Coimbra, Diogo P., Penedo, Diego M., Silva, Monique O.M., Abreu, Ana P.M., Silva, Cláudia B., Verona, Carlos E., Heliodoro, Gabriela C., Massard, Carlos L., Nogueira, Denise M.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-04-2020
Subjects:
DNA sequencing
Hemoparasite
Primates
Trypanosomatidae
Primates
Trypanosomatidae
Hemoparasite
DNA sequencing
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Summary:Callithrix jacchus and C. penicillata marmosets are invasive to the state of Rio de Janeiro, Brazil, threatening the native and vulnerable C. aurita. Both invasive species can be hosts of Trypanosoma cruzi, T. minasense, T. rangeli and T. devei. We aim to investigate the occurrence of trypanosomatids in Callithrix sp. from Jardim Botânico do Rio de Janeiro, located in a central and populous area of the city. Fifteen marmosets were captured. Blood samples were collected for light microscopy and molecular genetics analysis. Parasites morphometric values were evaluated for species identification. DNA was extracted from blood samples by phenol-chloroform method, for partial amplification of the 18S rRNA gene. PCR products were sequenced and aligned using BLAST®. A maximum likelihood phylogenetic tree was constructed to analyze the proximity between the observed sequences. By light microscopy, trypomastigotes were detected in five of the fifteen marmosets. Morphometric measurements and size polymorphism corresponded to those previously described for T. minasense. The DNA sequences of approximately 600 base pairs of the 18S rRNA gene were obtained for three samples with 99% identity with T. minasense sequence, forming a cluster in the phylogenetic tree and corroborating morphometric analysis. Trypanosoma minasense is a highly specific parasite to non-human primates considered as non-pathogenic. There is no evidence of infection in humans and these parasite findings from invasive marmosets do not support additional risks for the native species. [Display omitted] •Trypanosoma minasense in free-living marmosets Callithrix sp. from Rio de Janeiro – Brazil.•Morphometric identification of Trypanosoma minasense and its size polymorphism.•DNA amplification of the Trypanosoma sp.18S rRNA gene.
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ISSN:1383-5769
1873-0329
DOI:10.1016/j.parint.2019.101999