Histological evaluation of sheep ovarian tissue after laparoscopic partial ovariectomy

In the present study, there was assessment of the damage to tissue caused by partial laparoscopic ovariectomy using bipolar forceps in sheep. Fragments of ovaries of six sheep were removed using bipolar forceps by making a transverse section in the middle third of the organ via three-portals that we...

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Bibliographic Details
Published in:	Animal reproduction science Vol. 204; pp. 165 - 170
Main Authors:	Cunha Rossy, Kayan, Cordeiro Barbosa, Adriana Elizabeth, da Conceição Guilherme, Barbara, Guimarães Silva, Carla Rozilene, Pantoja Pereira, Carmen Silvia, Silva Ferreira, Chayanne, de Oliveira Bezerra, Daniella Kaísa, Melo Alves dos Santos, Gabriela, Soares da Costa, Filipe Luigui, Magno de Morais, Hanna Lyce, Barroso dos Santos, Janayna, da Silva Siqueira, Luciana, Pucci Bueno Borges, Luísa, da Silva Cavalcante, Maria Jeane, Santos Cunha, Michel, Abrantes de Oliveira, Renato, dos Santos Albuquerque, Rodrigo, Soares Bezerra Júnior, Pedro, Machado Silva, Marco Augusto, Maia Teixeira, Pedro Paulo
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-05-2019
Subjects:	Biopsy Bipolar forceps Laparoscopy Surgical methods Tissue conservation Laparoscopy Tissue conservation Biopsy Surgical methods Bipolar forceps
Online Access:	Get full text
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Summary:	In the present study, there was assessment of the damage to tissue caused by partial laparoscopic ovariectomy using bipolar forceps in sheep. Fragments of ovaries of six sheep were removed using bipolar forceps by making a transverse section in the middle third of the organ via three-portals that were made using laparoscopy. The fragments were subjected to standard histological examinations and the lesions attributed to the procedure were investigated using an optical microscope and Image J software. The results were assessed using an analysis of variance and the Tukey test. All the laminae had minimal tissue damage. The mean amount of highly damaged tissue was 1.8%, and of partially damaged tissue was 5.6%. The mean total area of healthy tissue in the fragments was 94.4%. The results of the study indicate this procedure can be conducted withvery little tissue damage occurring. The use of this procedure, therefore, can be incorporated in future reproductive studies without altering the functions of the in situ ovarian tissues.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-4320 1873-2232
DOI:	10.1016/j.anireprosci.2019.03.021