Effect of the Interaction Between Cryoprotectant Concentration and Cryopreservation Method on Frozen/Thawed Chicken Sperm Variables

This work examines the effect of the interaction between different concentrations of two cryoprotectants – glycerol (GLY) and dimethylacetamide (DMA) – and two methods of cryopreservation – pellets produced by plunging into liquid nitrogen and gradual in‐straw freezing – on frozen/thawed chicken spe...

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Published in:	Reproduction in domestic animals Vol. 50; no. 1; pp. 135 - 141
Main Authors:	Abouelezz, FMK, Castaño, C, Toledano‐Díaz, A, Esteso, MC, López‐Sebastián, A, Campo, JL, Santiago‐Moreno, J
Format:	Journal Article
Language:	English
Published:	Germany P. Parey Scientific Publishers 01-02-2015 Blackwell Publishing Ltd
Subjects:	Acetamides - administration & dosage acrosome Animal reproduction Animals Cell Survival Chickens Cryogenic engineering cryopreservation Cryopreservation - methods Cryopreservation - veterinary cryoprotectants Cryoprotective Agents - administration & dosage Dose-Response Relationship, Drug Fertility - drug effects freezing glycerol Glycerol - administration & dosage Hot Temperature Insemination, Artificial - veterinary Male nitrogen pellets Poultry Semen Preservation - methods Semen Preservation - veterinary Sperm Sperm Motility Spermatozoa - drug effects Spermatozoa - physiology viability
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Summary:	This work examines the effect of the interaction between different concentrations of two cryoprotectants – glycerol (GLY) and dimethylacetamide (DMA) – and two methods of cryopreservation – pellets produced by plunging into liquid nitrogen and gradual in‐straw freezing – on frozen/thawed chicken sperm variables. Sperm was cryopreserved using: (i) 6% DMA, following the in‐straw and the pellet methods (ii) 11% GLY, following the in‐straw and the pellet methods; and (iii) 8% GLY in the in‐straw method and 3% DMA in the pellet method (i.e. reduced cryoprotectant concentrations). When 6% DMA was used as the cryoprotectant, no differences were seen between the in‐straw and pellet methods in terms of frozen/thawed sperm variables or fertility (10.8% and 12.8%, respectively). The viability and motility variables of the frozen/thawed sperm produced using the in‐straw method with 11% GLY were higher (p < 0.05) than those recorded for the sperm preserved using the same cryoprotectant and concentration in the pellet method. However, fertility was extremely low in both groups (2.1% and 4.2% for the in‐straw and pellet methods, respectively). Finally, the use of 8% GLY in the in‐straw method returned higher sperm viability, intact acrosome and motility values than the use of 3% DMA in the pellet method (p < 0.01). No differences were seen, however, in the fertility results obtained (28.8% and 25.0%, respectively). These results suggest that cryoprotectant concentrations can be reduced and still provide acceptable fertility rates.
Bibliography:	http://dx.doi.org/10.1111/rda.12464 ArticleID:RDA12464 INIA - No. RZ2009-00001-C02; No. RZ2012-00013-C02 ark:/67375/WNG-LWC9DCMS-6 istex:95556CFB7A2C60474A22ED34C83F7A799FEE866F ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0936-6768 1439-0531
DOI:	10.1111/rda.12464