DNA Origami Nanopores

We demonstrate the assembly of functional hybrid nanopores for single molecule sensing by inserting DNA origami structures into solid-state nanopores. In our experiments, single artificial nanopores based on DNA origami are repeatedly inserted in and ejected from solid-state nanopores with diameters...

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Bibliographic Details
Published in:	Nano letters Vol. 12; no. 1; pp. 512 - 517
Main Authors:	Bell, Nicholas A. W, Engst, Christian. R, Ablay, Marc, Divitini, Giorgio, Ducati, Caterina, Liedl, Tim, Keyser, Ulrich F
Format:	Journal Article
Language:	English
Published:	Washington, DC American Chemical Society 11-01-2012
Subjects:	Assembly Condensed matter: structure, mechanical and thermal properties Cross-disciplinary physics: materials science; rheology Deoxyribonucleic acid Detection DNA - chemistry DNA - ultrastructure Ejection Exact sciences and technology General equipment and techniques Instruments, apparatus, components and techniques common to several branches of physics and astronomy Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties Materials science Materials Testing Methods of nanofabrication Molecular Probe Techniques Nanostructure Nanostructures - chemistry Nanostructures - ultrastructure Particle Size Physics Porosity Self assembly Sensors Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties) Ultrafiltration - methods biosensing resistive pulse sensing DNA origami single molecules Nanopores self assembly Self-assembly Sensors Nanopore DNA Nanoporosity
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Summary:	We demonstrate the assembly of functional hybrid nanopores for single molecule sensing by inserting DNA origami structures into solid-state nanopores. In our experiments, single artificial nanopores based on DNA origami are repeatedly inserted in and ejected from solid-state nanopores with diameters around 15 nm. We show that these hybrid nanopores can be employed for the detection of λ-DNA molecules. Our approach paves the way for future development of adaptable single-molecule nanopore sensors based on the combination of solid-state nanopores and DNA self-assembly.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1530-6984 1530-6992
DOI:	10.1021/nl204098n