Free Radical Scavenging Activity of Aqueous and Ethanolic Extract of Brassica oleracea L. var. italica

In this study, antioxidant activities of aqueous and ethanolic extracts of Brassica oleracea L. var. italica were investigated. The antioxidant properties of both extracts of Brassica oleracea L. var. italica were evaluated using different antioxidant tests, including 1, 1-diphenyl-2-picrylhydrazyl...

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Bibliographic Details
Published in:	Food and bioprocess technology Vol. 4; no. 7; pp. 1137 - 1143
Main Authors:	Bidchol, Abdul Mueed, Wilfred, A., Abhijna, P., Harish, R.
Format:	Journal Article
Language:	English
Published:	New York Springer-Verlag 01-10-2011 Springer Nature B.V
Subjects:	Acetic acid Agriculture Animal models Antioxidants Biotechnology Brassica Brassica oleracea Chelation Chemistry Chemistry and Materials Science Chemistry/Food Science Ethylenediamine Food Science Free radicals Functional foods & nutraceuticals In vivo methods and tests Lipid peroxidation Lipids Metal ions Original Paper Oxidation Oxidative stress Peroxidation Reduction (metal working) Scavenging Superoxide anions Lipid peroxidation L. var Reactive oxygen species Metal ion chelation Antioxidant activity
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Summary:	In this study, antioxidant activities of aqueous and ethanolic extracts of Brassica oleracea L. var. italica were investigated. The antioxidant properties of both extracts of Brassica oleracea L. var. italica were evaluated using different antioxidant tests, including 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, superoxide radical scavenging, inhibition of microsomal lipid peroxidation, reduction of power, and metal ion chelating activities. Inhibition of superoxide scavenging by aqueous and ethanolic extracts showed an IC 50 of 0.93 and 0.25 mg/ml, respectively. Metal ion chelation showed an IC 50 of 0.35 mg/ml of both the extracts and was equipotent to positive control, ethylenediamine tetra-acetic acid. The ethanolic extract of Brassica oleracea L. var. italica exhibited higher antioxidant activity in DPPH radical and superoxide anion scavenging than that of aqueous extract. The results obtained in the in vitro models clearly suggest that, Brassica oleracea L. var. italica is a natural source for antioxidants, which could serve as a nutraceutical with potential applications in reducing the level of oxidative stress and related health benefits. However, comprehensive studies need to be conducted to ascertain the in vivo safety of such extracts in experimental animal models.
ISSN:	1935-5130 1935-5149
DOI:	10.1007/s11947-009-0196-9