Search Results - "Abellanas‐Perez, Pedro"
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The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support
Published in Biotechnology progress (01-01-2024)“…In this article, we have analyzed the interactions between enzyme crowding on a given support and its chemical modification (ethylenediamine modification via…”
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G Protein-Coupled Estrogen Receptor Immunoreactivity Fluctuates During the Estrous Cycle and Show Sex Differences in the Amygdala and Dorsal Hippocampus
Published in Frontiers in endocrinology (Lausanne) (07-08-2020)“…G protein-coupled estrogen receptor (GPER) in the amygdala and the dorsal hippocampus mediates actions of estradiol on anxiety, social recognition and spatial…”
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Support Enzyme Loading Influences the Effect of Aldehyde Dextran Modification on the Specificity of Immobilized Ficin for Large Proteins
Published in Molecules (Basel, Switzerland) (01-08-2024)“…It has been reported that the modification of immobilized glyoxyl-ficin with aldehyde dextran can promote steric hindrances that greatly reduce the activity of…”
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The Effects of Buffer Nature on Immobilized Lipase Stability Depend on Enzyme Support Loading
Published in Catalysts (01-01-2024)“…The lipases from Thermomyces lanuginosus (TLL) and Candida antarctica (B) (CALB) were immobilized on octyl-agarose beads at 1 mg/g (a loading under the…”
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Tuning Almond Lipase Features by Using Different Immobilization Supports
Published in Catalysts (01-01-2024)“…The lipase from Prunus dulcis almonds has been immobilized for the first time. For this purpose, two different supports, an octadecyl methacrylate particulate…”
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Glutaraldehyde modification of lipases immobilized on octyl agarose beads: Roles of the support enzyme loading and chemical amination of the enzyme on the final enzyme features
Published in International journal of biological macromolecules (01-09-2023)“…Lipase B from Candida antarctica (CALB) and lipase from Thermomyces lanuginosus (TLL) have been immobilized on octyl agarose at low loading and at a loading…”
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The nature of the buffer alters the effects of the chemical modification on the stability of immobilized lipases
Published in Process biochemistry (1991) (01-10-2023)“…The objective of this paper was to analyze whether an interaction between the effects of the buffer nature and chemical modification on enzyme stability…”
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Tailoring the specificity of ficin versus large hemoglobin and small casein by co-immobilizing inert proteins on the immobilized enzyme layer and further modification with aldehyde dextran
Published in International journal of biological macromolecules (01-10-2024)“…Ficin has been immobilized at full loading on glyoxyl agarose beads. Then, ficin was blocked with 2,2′-dipyridyldisulfide. To be effective, the modification…”
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Designing tailor-made steric matters to improve the immobilized ficin specificity for small versus large proteins
Published in Journal of biotechnology (20-11-2024)“…The development of strategies that can permit to adjust the size specificity of immobilized proteases by the generation of steric hindrances may enlarge its…”
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Tuning almond lipase features by the buffer used during immobilization: The apparent biocatalysts stability depends on the immobilization and inactivation buffers and the substrate utilized
Published in Journal of biotechnology (10-08-2024)“…The lipase from Prunus dulcis almonds was inactivated under different conditions. At pH 5 and 9, enzyme stability remained similar under the different studied…”
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11
Optimizing the activation of agarose beads with divinyl sulfone for enzyme immobilization and stabilization
Published in International journal of biological macromolecules (01-12-2024)“…The focus of the present work is to find the optimal conditions for the activation of agarose beads with divinyl sulfone (DVS). The reactivity of the vinyl…”
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A review on the immobilization of bromelain
Published in International journal of biological macromolecules (01-07-2024)“…This review shows the endeavors performed to prepare immobilized formulations of bromelain extract, usually from pineapple, and their use in diverse…”
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Reutilization of the Most Stable Coimmobilized Enzyme Using Glutaraldehyde Chemistry to Produce a New Combi-biocatalyst When the Coimmobilized Enzyme with a Lower Stability Is Inactivated
Published in ACS sustainable chemistry & engineering (29-04-2024)“…In the present article, glutaraldehyde was used to covalently coimmobilize the lipase Eversa Transform 2.0 and the β-galactosidase from Aspergillus oryzae…”
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