Characterization and phylogeny of fungi isolated from industrial wastewater using multiple genes

The aim of this study was the isolation and molecular characterization of fungi from untreated refinery effluent by using multiple conserved genes. The Fungi isolated were characterized based on PCR amplification and genomic sequencing of the internal transcribed spacer region (ITS), partial β-tubul...

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Bibliographic Details
Published in:	Scientific reports Vol. 12; no. 1; p. 2094
Main Authors:	Ezeonuegbu, Blessing Amaka, Abdullahi, Machido Dauda, Whong, Clement M. Z., Sohunago, Japhet Wisdom, Kassem, Hazem S., Yaro, Clement Ameh, Hetta, Helal F., Mostafa-Hedeab, Gomaa, Zouganelis, George D., Batiha, Gaber El-Saber
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 08-02-2022 Nature Publishing Group Nature Portfolio
Subjects:	631/158 631/326 Aspergillus Calcium-binding protein Calmodulin Calmodulin - genetics DNA, Intergenic DNA-directed RNA polymerase Fungi Fungi - genetics Fungi - isolation & purification Genes, Fungal Humanities and Social Sciences Industrial wastewater multidisciplinary Oil and Gas Industry Penicillium Phylogeny RNA polymerase RNA Polymerase II - genetics Science Science (multidisciplinary) Spacer region Species Tubulin Tubulin - genetics Waste Water - microbiology Wastewater
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Summary:	The aim of this study was the isolation and molecular characterization of fungi from untreated refinery effluent by using multiple conserved genes. The Fungi isolated were characterized based on PCR amplification and genomic sequencing of the internal transcribed spacer region (ITS), partial β-tubulin (BenA), calmodulin (CaM), and RNA polymerase second large subunit (RPB2) genes, along with morphological characterization. The obtained sequences were subjected to BLAST analysis and the corresponding fungal isolates were assigned species names after comparison with representative sequences available in GenBank. Fifteen (15) Fungi species belonging to four genera of Aspergillus , Penicillium , Fusarium , and Trichoderma with Aspergillus as the predominant genus were identified. Therefore these genes should be used as molecular markers for species level identification of fungi (especially Aspergillus and Penicillium as proven in this study.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-022-05820-9