Levels of Ancylostoma infections and phylogenetic analysis of cox 1 gene of A. ceylanicum in stray cat faecal samples from Guangzhou, China

Levels of Ancylostoma infections and phylogenetic analysis of cox 1 gene of A. ceylanicum in stray cat faecal samples from Guangzhou, China

Ancylostoma ceylanicum is a common zoonotic nematode. Cats act as natural reservoirs of the hookworm and are involved in transmitting infection to humans, thus posing a potential risk to public health. The prevalence of feline A. ceylanicum in Guangzhou (South China) was surveyed by polymerase chain...

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Bibliographic Details
Published in:Journal of helminthology Vol. 90; no. 4; p. 392
Main Authors: Hu, W, Yu, X G, Wu, S, Tan, L P, Song, M R, Abdulahi, A Y, Wang, Z, Jiang, B, Li, G Q
Format: Journal Article
Language:English
Published: England 01-07-2016
Subjects:
Ancylostoma - classification
Ancylostoma - genetics
Ancylostoma - isolation & purification
Ancylostomiasis - epidemiology
Ancylostomiasis - parasitology
Ancylostomiasis - veterinary
Animals
Cat Diseases - epidemiology
Cat Diseases - parasitology
Cats
China - epidemiology
Cluster Analysis
Cyclooxygenase 1 - genetics
Feces - parasitology
Genetic Variation
Genotype
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Prevalence
Sequence Analysis, DNA
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Summary:Ancylostoma ceylanicum is a common zoonotic nematode. Cats act as natural reservoirs of the hookworm and are involved in transmitting infection to humans, thus posing a potential risk to public health. The prevalence of feline A. ceylanicum in Guangzhou (South China) was surveyed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In total, 112 faecal samples were examined; 34.8% (39/112) and 43.8% (49/112) samples were positive with hookworms by microscopy and PCR method, respectively. Among them, 40.8% of samples harboured A. ceylanicum. Twelve positive A. ceylanicum samples were selected randomly and used for cox 1 sequence analysis. Sequencing results revealed that they had 97-99% similarity with A. ceylanicum cox 1 gene sequences deposited in GenBank. A phylogenetic tree showed that A. ceylanicum isolates were divided into two groups: one comprising four isolates from Guangzhou (South China), and the other comprising those from Malaysia, Cambodia and Guangzhou. In the latter group, all A. ceylanicum isolates from Guangzhou were clustered into a minor group again. The results indicate that the high prevalence of A. ceylanicum in stray cats in South China poses a potential risk of hookworm transmission from pet cats to humans, and that A. ceylanicum may be a species complex worldwide.
ISSN:1475-2697
DOI:10.1017/S0022149X15000413