The founder-cell transcriptome in the Arabidopsis apetala1 cauliflower inflorescence meristem

Although the pattern of lateral organ formation from apical meristems establishes species-specific plant architecture, the positional information that confers cell fate to cells as they transit to the meristem flanks where they differentiate, remains largely unknown. We have combined fluorescence-ac...

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Bibliographic Details
Published in:	BMC genomics Vol. 17; no. 1; p. 855
Main Authors:	Frerichs, Anneke, Thoma, Rahere, Abdallah, Ali Taleb, Frommolt, Peter, Werr, Wolfgang, Chandler, John William
Format:	Journal Article
Language:	English
Published:	England BioMed Central 03-11-2016
Subjects:	Brassica - genetics Cluster Analysis Computational Biology - methods Epigenesis, Genetic Gene Expression Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Gene Ontology Gene Regulatory Networks Genes, Reporter Genomics Inflorescence - genetics Meristem - genetics Phenotype Plant Cells - metabolism RNA Processing, Post-Transcriptional Transcriptome Fluorescence-activated cell sorting apetala1 cauliflower RNA-seq Bract Transcriptome Lateral organ founder cell DORNRÖSCHEN-LIKE Inflorescence meristem
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Summary:	Although the pattern of lateral organ formation from apical meristems establishes species-specific plant architecture, the positional information that confers cell fate to cells as they transit to the meristem flanks where they differentiate, remains largely unknown. We have combined fluorescence-activated cell sorting and RNA-seq to characterise the cell-type-specific transcriptome at the earliest developmental time-point of lateral organ formation using DORNRÖSCHEN-LIKE::GFP to mark founder-cell populations at the periphery of the inflorescence meristem (IM) in apetala1 cauliflower double mutants, which overproliferate IMs. Within the lateral organ founder-cell population at the inflorescence meristem, floral primordium identity genes are upregulated and stem-cell identity markers are downregulated. Additional differentially expressed transcripts are involved in polarity generation and boundary formation, and in epigenetic and post-translational changes. However, only subtle transcriptional reprogramming within the global auxin network was observed. The transcriptional network of differentially expressed genes supports the hypothesis that lateral organ founder-cell specification involves the creation of polarity from the centre to the periphery of the IM and the establishment of a boundary from surrounding cells, consistent with bract initiation. However, contrary to the established paradigm that sites of auxin response maxima pre-pattern lateral organ initiation in the IM, auxin response might play a minor role in the earliest stages of lateral floral initiation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1471-2164 1471-2164
DOI:	10.1186/s12864-016-3189-x