Search Results - "Aach, John"

RNA-Guided Human Genome Engineering via Cas9 by Mali, Prashant, Yang, Luhan, Esvelt, Kevin M., Aach, John, Guell, Marc, DiCarlo, James E., Norville, Julie E., Church, George M.

“…Bacteria and archaea have evolved adaptive immune defenses, termed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas)…”
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CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering by Mali, Prashant, Aach, John, Stranges, P Benjamin, Esvelt, Kevin M, Moosburner, Mark, Kosuri, Sriram, Yang, Luhan, Church, George M

“…A screen in human cells defines the targeting specificities of sgRNA:Cas9 and TAL-based transcriptional activators. Prokaryotic type II CRISPR-Cas systems can…”
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Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9 by Li, Jian-Feng, Norville, Julie E, Aach, John, McCormack, Matthew, Zhang, Dandan, Bush, Jenifer, Church, George M, Sheen, Jen

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Genome-wide inactivation of porcine endogenous retroviruses (PERVs) by Yang, Luhan, Güell, Marc, Niu, Dong, George, Haydy, Lesha, Emal, Grishin, Dennis, Aach, John, Shrock, Ellen, Xu, Weihong, Poci, Jürgen, Cortazio, Rebeca, Wilkinson, Robert A., Fishman, Jay A., Church, George

“…The shortage of organs for transplantation is a major barrier to the treatment of organ failure. Although porcine organs are considered promising, their use…”
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Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues by Lee, Je Hyuk, Daugharthy, Evan R, Scheiman, Jonathan, Kalhor, Reza, Ferrante, Thomas C, Terry, Richard, Turczyk, Brian M, Yang, Joyce L, Lee, Ho Suk, Aach, John, Zhang, Kun, Church, George M

“…Lee et al . provide their protocol for fluorescent in situ sequencing (FISSEQ) of RNA. This technique allows spatial and quantitative information about mRNA…”
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Highly efficient Cas9-mediated transcriptional programming by Chavez, Alejandro, Scheiman, Jonathan, Vora, Suhani, Pruitt, Benjamin W, Tuttle, Marcelle, P R Iyer, Eswar, Lin, Shuailiang, Kiani, Samira, Guzman, Christopher D, Wiegand, Daniel J, Ter-Ovanesyan, Dmitry, Braff, Jonathan L, Davidsohn, Noah, Housden, Benjamin E, Perrimon, Norbert, Weiss, Ron, Aach, John, Collins, James J, Church, George M

“…The fusion of three transcriptional activation domains to a nuclease-deficient Cas9 achieves robust induction of gene expression and can induce differentiation…”
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Quantitative Morphological Signatures Define Local Signaling Networks Regulating Cell Morphology by Bakal, Chris, Aach, John, Church, George, Perrimon, Norbert

“…Although classical genetic and biochemical approaches have identified hundreds of proteins that function in the dynamic remodeling of cell shape in response to…”
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Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells by Byrne, Susan M, Ortiz, Luis, Mali, Prashant, Aach, John, Church, George M

“…Sequence-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far been used to disrupt, correct or insert transgenes at precise locations in…”
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Highly Multiplexed Subcellular RNA Sequencing in Situ by Lee, Je Hyuk, Daugharthy, Evan R., Scheiman, Jonathan, Kalhor, Reza, Yang, Joyce L., Ferrante, Thomas C., Terry, Richard, Jeanty, Sauveur S. F., Li, Chao, Amamoto, Ryoji, Peters, Derek T., Turczyk, Brian M., Marblestone, Adam H., Inverso, Samuel A., Bernard, Amy, Mali, Prashant, Rios, Xavier, Aach, John, Church, George M.

“…Understanding the spatial organization of gene expression with single-nucleotide resolution requires localizing the sequences of expressed RNA transcripts…”
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Addressing the ethical issues raised by synthetic human entities with embryo-like features by Aach, John, Lunshof, Jeantine, Iyer, Eswar, Church, George M

“…The "14-day rule" for embryo research stipulates that experiments with intact human embryos must not allow them to develop beyond 14 days or the appearance of…”
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Optimization of scarless human stem cell genome editing by Yang, Luhan, Guell, Marc, Byrne, Susan, Yang, Joyce L, De Los Angeles, Alejandro, Mali, Prashant, Aach, John, Kim-Kiselak, Caroline, Briggs, Adrian W, Rios, Xavier, Huang, Po-Yi, Daley, George, Church, George

“…Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs) will enable sophisticated genome engineering for research and…”
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Chromosome-scale, haplotype-resolved assembly of human genomes by Garg, Shilpa, Fungtammasan, Arkarachai, Carroll, Andrew, Chou, Mike, Schmitt, Anthony, Zhou, Xiang, Mac, Stephen, Peluso, Paul, Hatas, Emily, Ghurye, Jay, Maguire, Jared, Mahmoud, Medhat, Cheng, Haoyu, Heller, David, Zook, Justin M., Moemke, Tobias, Marschall, Tobias, Sedlazeck, Fritz J., Aach, John, Chin, Chen-Shan, Church, George M., Li, Heng

“…Haplotype-resolved or phased genome assembly provides a complete picture of genomes and their complex genetic variations. However, current algorithms for…”
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Genome-Wide Identification of Human RNA Editing Sites by Parallel DNA Capturing and Sequencing by Li, Jin Billy, Levanon, Erez Y., Yoon, Jung-Ki, Aach, John, Xie, Bin, LeProust, Emily, Zhang, Kun, Gao, Yuan, Church, George M.

“…Adenosine-to-inosine (A-to-I) RNA editing leads to transcriptome diversity and is important for normal brain function. To date, only a handful of functional…”
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Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells by Yang, Luhan, Grishin, Dennis, Wang, Gang, Aach, John, Zhang, Cheng-Zhong, Chari, Raj, Homsy, Jason, Cai, Xuyu, Zhao, Yue, Fan, Jian-Bing, Seidman, Christine, Seidman, Jonathan, Pu, William, Church, George

“…CRISPR/Cas9 has demonstrated a high-efficiency in site-specific gene targeting. However, potential off-target effects of the Cas9 nuclease represent a major…”
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Engineering and optimising deaminase fusions for genome editing by Yang, Luhan, Briggs, Adrian W., Chew, Wei Leong, Mali, Prashant, Guell, Marc, Aach, John, Goodman, Daniel Bryan, Cox, David, Kan, Yinan, Lesha, Emal, Soundararajan, Venkataramanan, Zhang, Feng, Church, George

“…Precise editing is essential for biomedical research and gene therapy. Yet, homology-directed genome modification is limited by the requirements for genomic…”
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Optimizing complex phenotypes through model-guided multiplex genome engineering by Kuznetsov, Gleb, Goodman, Daniel B, Filsinger, Gabriel T, Landon, Matthieu, Rohland, Nadin, Aach, John, Lajoie, Marc J, Church, George M

“…We present a method for identifying genomic modifications that optimize a complex phenotype through multiplex genome engineering and predictive modeling. We…”
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Rational optimization of tolC as a powerful dual selectable marker for genome engineering by Gregg, Christopher J, Lajoie, Marc J, Napolitano, Michael G, Mosberg, Joshua A, Goodman, Daniel B, Aach, John, Isaacs, Farren J, Church, George M

“…Selection has been invaluable for genetic manipulation, although counter-selection has historically exhibited limited robustness and convenience. TolC, an…”
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Proteome-wide systems analysis of a cellulosic biofuel-producing microbe by Tolonen, Andrew C, Haas, Wilhelm, Chilaka, Amanda C, Aach, John, Gygi, Steven P, Church, George M

“…Fermentation of plant biomass by microbes like Clostridium phytofermentans recycles carbon globally and can make biofuels from inedible feedstocks. We analyzed…”
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Improved cell-free RNA and protein synthesis system by Li, Jun, Gu, Liangcai, Aach, John, Church, George M

“…Cell-free RNA and protein synthesis (CFPS) is becoming increasingly used for protein production as yields increase and costs decrease. Advances in…”
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Identification of molecular markers of bipolar cells in the murine retina by Kim, Douglas S., Ross, Sarah E., Trimarchi, Jeffrey M., Aach, John, Greenberg, Michael E., Cepko, Constance L.

“…Retinal bipolar neurons serve as relay interneurons that connect rod and cone photoreceptor cells to amacrine and ganglion cells. They exhibit diverse…”
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