Search Results - "ATHA, D. H"

Standardization of PCR amplification for fragile X trinucleotide repeat measurements by O'Connell, CD, Atha, DH, Jakupciak, JP, Amos, JA, Richie, Kl

“…To provide the clinical diagnostics community with accurate protocols and measurements for the detection of genetic disorders, we have established a…”
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Prediction of DNA single-strand conformation polymorphism: analysis by capillary electrophoresis and computerized DNA modeling by Atha, D H, Kasprzak, W, O'Connell, C D, Shapiro, B A

“…We have analyzed previously three representative p53 single-point mutations by capillary-electrophoresis single-strand conformation polymorphism (CE-SSCP). In…”
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Cloned bovine aortic endothelial cells synthesize anticoagulantly active heparan sulfate proteoglycan by Marcum, J A, Atha, D H, Fritze, L M, Nawroth, P, Stern, D, Rosenberg, R D

“…Cloned bovine aortic endothelial cells were cultured with [35S]Na2SO4 and proteolyzed extensively with papain. Radiolabeled heparan sulfate was isolated by…”
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Improved methods and standards for telomerase detection: quantitative histopathology using antibody staining by Jakupciak, J P, Gallant, N D, Smith, A H, Becker, M L, Tona, A, Atha, D H

“…Evaluation of telomerase as an early detection biomarker for cancer has been hindered by a lack of reliable methods and standards for in situ histochemical…”
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Mechanism of precipitation of proteins by polyethylene glycols. Analysis in terms of excluded volume by Atha, D H, Ingham, K C

“…The apparent solubilities of various proteins (14,000 to 670,000 daltons) were measured in the presence of polyethylene glycols (PEGs) of different sizes. All…”
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Interaction of heparin with fibronectin and isolated fibronectin domains by Ingham, K C, Brew, S A, Atha, D H

“…Fluorescence polarization, gel exclusion chromatography and affinity chromatography were used to characterize the interaction of heparins of different size…”
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Contribution of monosaccharide residues in heparin binding to antithrombin III by Atha, Donald H, Lormeau, Jean Claude, Petitou, Maurice, Rosenberg, Robert D, Choay, Jean

“…The importance of 3-O- and 6-O-sulfated glucosamine residues within the heparin octasaccharide iduronic acid(1)---N-acetylglucosamine…”
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Evaluation of Critical Groups Required for the Binding of Heparin to Antithrombin by Atha, Donald H., Stephens, Andrew W., Rosenberg, Robert D.

“…We have examined the quantitative importance of various monosaccharide residues of an octasaccharide domain of heparin that are responsible for the binding of…”
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Structural analysis of heparin by raman spectroscopy by Atha, D H, Gaigalas, A K, Reipa, V

“…The Raman spectra of commercially available heparin disaccharide standards exhibit bands associated with the N-sulfate and the 6-O-sulfate groups of the…”
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Identification of known p53 point mutations by capillary electrophoresis using unique mobility profiles in a blinded study by WENZ, H. M, RAMACHANDRA, S, O'CONNELL, C. D, ATHA, D. H

“…This study is part of an ongoing project at the National Institute of Standards and Technology (NIST) that generates a panel of DNA clones containing the most…”
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Expression and characterization of human antithrombin III synthesized in mammalian cells by Wasley, L C, Atha, D H, Bauer, K A, Kaufman, R J

“…Antithrombin III (ATIII) has been expressed in transiently transfected COS-1 monkey cells and in stably transformed Chinese hamster ovary cells, and the…”
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Physical characterization of heparin by light scattering by Gaigalas, A K, Hubbard, J B, LeSage, R, Atha, D H

“…Dynamic and electrophoretic light scattering were used to measure the size and charge heterogeneity of a commercial preparation of heparin. For this…”
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Thermal denaturation of antithrombin III. Stabilization by heparin and lyotropic anions by Busby, T F, Atha, D H, Ingham, K C

“…Antithrombin III is of potential value for replacement therapy in patients with acquired or congenital deficiencies. Pasteurization of the purified inhibitor…”
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Sequence variation in heparin octasaccharides with high affinity for antithrombin III by Atha, Donald H, Stephens, Andrew W, Rimon, Abraham, Rosenberg, Robert D

“…We have isolated from nitrous acid cleavage products of heparin two major octasaccharide fragments which bind with high affinity to human antithrombin…”
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Detection of p53 point mutations by single strand conformation polymorphism: analysis by capillary electrophoresis by Atha, D H, Wenz, H M, Morehead, H, Tian, J, O'Connell, C D

“…We have analyzed five p53 single point mutations by single strand conformation polymorphism using capillary electrophoresis (CE-SSCP) and have compared these…”
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Analysis of environment-induced DNA damage by capillary electrophoresis by Nackerdien, Z E, Siles, B A, Nevins, S A, Atha, D H

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SSCP analysis of point mutations by multicolor capillary electrophoresis by Hayashi, K, Wenz, H M, Inazuka, M, Tahira, T, Sasaki, T, Atha, D H

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Characterization of DNA standards by capillary electrophoresis by Atha, D H

“…We have used capillary electrophoresis to evaluate commercial DNA size standards and have found that it can provide an efficient assessment of size. However,…”
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Separation and characterization of fibronectin domains by two-dimensional electrophoresis by Edwards, J J, Reeder, D J, Atha, D H

“…Two-dimensional gel electrophoresis (2DGE) and image processing were used to quantify protein and carbohydrate heterogeneity in human plasma fibronectin (FN)…”
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Thermodynamic analysis of heparin binding to human antithrombin by DeLauder, S, Schwarz, F P, Williams, Sr, J C, Atha, D H

“…The binding of heparin to human antithrombin III (ATIII) was investigated by titration calorimetry (TC) and differential scanning calorimetry (DSC). TC…”
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