Stimulatory effects of benzene on rabbit liver and kidney microsomal cytochrome P-450 dependent drug metabolizing enzymes

Treatment of rabbits with benzene (880 mg/kg/day), s.c. for 3 consecutive days, caused 3.8- and 5.7-fold increases in aniline 4-hydroxylation rates of liver and kidney microsomes, respectively. Benzene treatment markedly enhanced hydroxylation rats of p-nitrophenol by liver and kidney by 7.2- and 4....

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Bibliographic Details
Published in:	Archives of toxicology Vol. 65; no. 3; pp. 186 - 190
Main Authors:	ARMC, E, ADALI, O, ISCAN, M, GUÊRAY, T
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-01-1991
Subjects:	Animals Benzene - toxicity Biological and medical sciences Chemical and industrial products toxicology. Toxic occupational diseases Cytochrome P-450 Enzyme System - analysis Electrophoresis, Polyacrylamide Gel Kidney - drug effects Kidney - enzymology Lung - drug effects Lung - enzymology Male Medical sciences Microsomes - drug effects Microsomes - enzymology Microsomes, Liver - drug effects Microsomes, Liver - enzymology Pharmaceutical Preparations - metabolism Rabbits Solvents Toxicology Enzyme Isozyme Cytochrome P450 Liver Rabbit Tissue specificity Lagomorpha Kidney Vertebrata Mammalia Animal Benzene Drug-metabolizing enzyme Subcutaneous administration
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Summary:	Treatment of rabbits with benzene (880 mg/kg/day), s.c. for 3 consecutive days, caused 3.8- and 5.7-fold increases in aniline 4-hydroxylation rates of liver and kidney microsomes, respectively. Benzene treatment markedly enhanced hydroxylation rats of p-nitrophenol by liver and kidney by 7.2- and 4.2-fold, respectively. Both of these enzymes are associated with cytochrome P-450 LM3a. In contrast, the activity of benzphetamine N-demethylase, associated with P-450 LM2, was not altered significantly in either liver or kidney microsomes. Although the total cytochrome P-450 contents of liver and kidney microsomes were not altered significantly by the benzene treatment, in the case of liver microsomes, formation of a new cytochrome P-450 with an apparent Mr of 51,400 was observed on SDS-PAGE. On the other hand, in the kidney microsomes, the intensity of the bands corresponding to approximate Mr of 50,000 and 51,400 was markedly increased. The results of the present work, in combination with those of the previous work (Arinç et al. 1988), indicate the existence of tissue specificity in the induction of rabbit P-450 isozyme by benzene.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0340-5761 1432-0738
DOI:	10.1007/BF02307307