Search Results - "ANNAN, Roland S"

Hydrophilic Interaction Chromatography Reduces the Complexity of the Phosphoproteome and Improves Global Phosphopeptide Isolation and Detection by McNulty, Dean E., Annan, Roland S.

“…The diversity and complexity of proteins and peptides in biological systems requires powerful liquid chromatography-based separations to optimize resolution…”
Get full text

Advances in high‐throughput mass spectrometry in drug discovery by Dueñas, Maria Emilia, Peltier‐Heap, Rachel E, Leveridge, Melanie, Annan, Roland S, Büttner, Frank H, Trost, Matthias

“…High‐throughput (HT) screening drug discovery, during which thousands or millions of compounds are screened, remains the key methodology for identifying active…”
Get full text

MAPK Scaffold IQGAP1 Binds the EGF Receptor and Modulates Its Activation by McNulty, Dean E., Li, Zhigang, White, Colin D., Sacks, David B., Annan, Roland S.

“…Cellular responses produced by EGF are mediated through the receptor (EGFR) and by various enzymes and scaffolds. Recent studies document IQGAP1 as a scaffold…”
Get full text

Structure-Based Design of a Novel SMYD3 Inhibitor that Bridges the SAM-and MEKK2-Binding Pockets by Van Aller, Glenn S., Graves, Alan P., Elkins, Patricia A., Bonnette, William G., McDevitt, Patrick J., Zappacosta, Francesca, Annan, Roland S., Dean, Tony W., Su, Dai-Shi, Carpenter, Christopher L., Mohammad, Helai P., Kruger, Ryan G.

“…SMYD3 is a lysine methyltransferase overexpressed in colorectal, breast, prostate, and hepatocellular tumors, and has been implicated as an oncogene in human…”
Get full text

Antitumor activity of an allosteric inhibitor of centromere-associated protein-E by Wood, Kenneth W, Lad, Latesh, Luo, Lusong, Qian, Xiangping, Knight, Steven D, Nevins, Neysa, Brejc, Katjusa, Sutton, David, Gilmartin, Aidan G, Chua, Penelope R, Desai, Radhika, Schauer, Stephen P, McNulty, Dean E, Annan, Roland S, Belmont, Lisa D, Garcia, Carlos, Lee, Yan, Diamond, Melody A, Faucette, Leo F, Giardiniere, Michele, Zhang, ShuYun, Sun, Chiu-Mei, Vidal, Justin D, Lichtsteiner, Serge, Cornwell, William D, Greshock, Joel D, Wooster, Richard F, Finer, Jeffrey T, Copeland, Robert A, Huang, Pearl S, Morgans, David J. Jr, Dhanak, Dashyant, Bergnes, Gustave, Sakowicz, Roman, Jackson, Jeffrey R

“…Centromere-associated protein-E (CENP-E) is a kinetochore-associated mitotic kinesin that is thought to function as the key receptor responsible for mitotic…”
Get full text

Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissues by Noto, Paul B., Sikorski, Timothy W., Zappacosta, Francesca, Wagner, Craig D., Montes de Oca, Rocio, Szapacs, Matthew E., Annan, Roland S., Liu, Yan, McHugh, Charles F., Mohammad, Helai P., Piccoli, Steven P., Creasy, Caretha L.

“…Arginine methylation has been recognized as a post-translational modification with pleiotropic effects that span from regulation of transcription to metabolic…”
Get full text

An Optimized Platform for Hydrophilic Interaction Chromatography–Immobilized Metal Affinity Chromatography Enables Deep Coverage of the Rat Liver Phosphoproteome by Zappacosta, Francesca, Scott, Gilbert F, Huddleston, Michael J, Annan, Roland S

“…While analysis of the phosphoproteome has become an important component of understanding how cells function, it remains a nontrivial task in terms of the…”
Get full text

Complement membrane attack complex is an immunometabolic regulator of NLRP3 activation and IL-18 secretion in human macrophages by Jimenez-Duran, Gisela, Kozole, Joseph, Peltier-Heap, Rachel, Dickinson, Eleanor R., Kwiatkowski, Christopher R., Zappacosta, Francesca, Annan, Roland S., Galwey, Nicholas W., Nichols, Eva-Maria, Modis, Louise K., Triantafilou, Martha, Triantafilou, Kathy, Booty, Lee M.

“…The complement system is an ancient and critical part of innate immunity. Recent studies have highlighted novel roles of complement beyond lysis of invading…”
Get full text

Dbf2-Mob1 Drives Relocalization of Protein Phosphatase Cdc14 to the Cytoplasm during Exit from Mitosis by Mohl, Dane A., Huddleston, Michael J., Collingwood, Therese S., Annan, Roland S., Deshaies, Raymond J.

“…Exit from mitosis is characterized by a precipitous decline in cyclin-dependent kinase (Cdk) activity, dissolution of mitotic structures, and cytokinesis. In…”
Get full text

Quinoline 3-sulfonamides inhibit lactate dehydrogenase A and reverse aerobic glycolysis in cancer cells by Billiard, Julia, Dennison, Jennifer B, Briand, Jacques, Annan, Roland S, Chai, Deping, Colón, Mariela, Dodson, Christopher S, Gilbert, Seth A, Greshock, Joel, Jing, Junping, Lu, Hong, McSurdy-Freed, Jeanelle E, Orband-Miller, Lisa A, Mills, Gordon B, Quinn, Chad J, Schneck, Jessica L, Scott, Gilbert F, Shaw, Anthony N, Waitt, Gregory M, Wooster, Richard F, Duffy, Kevin J

“…Most normal cells in the presence of oxygen utilize glucose for mitochondrial oxidative phosphorylation. In contrast, many cancer cells rapidly convert glucose…”
Get full text

A high-throughput MALDI-TOF MS biochemical screen for small molecule inhibitors of the antigen aminopeptidase ERAP1 by Müller, Leonie, Burton, Amy K, Tayler, Chloe L, Rowedder, James E, Hutchinson, Jonathan P, Peace, Simon, Quayle, Julie M, Leveridge, Melanie V, Annan, Roland S, Trost, Matthias, Peltier-Heap, Rachel E, Dueñas, Maria Emilia

“…MALDI-TOF MS is a powerful analytical technique that provides a fast and label-free readout for in vitro assays in the high-throughput screening (HTS)…”
Get full text

Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus by Azzam, R, Chen, S.L, Shou, W, Mah, A.S, Alexandru, G, Nasmyth, K, Annan, R.S, Carr, S.A, Deshaies, R.J

“…Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into…”
Get full text

Biochemical characterization of human HIF hydroxylases using HIF protein substrates that contain all three hydroxylation sites by Pappalardi, Melissa B, McNulty, Dean E, Martin, John D, Fisher, Kelly E, Jiang, Yong, Burns, Matthew C, Zhao, Huizhen, Ho, Thau, Sweitzer, Sharon, Schwartz, Benjamin, Annan, Roland S, Copeland, Robert A, Tummino, Peter J, Luo, Lusong

“…The HIF (hypoxia-inducible factor) plays a central regulatory role in oxygen homoeostasis. HIF proteins are regulated by three Fe(II)- and α-KG…”
Get more information

Mammalian Chk2 is a downstream effector of the ATM-dependent DNA damage checkpoint pathway by CHATURVEDI, P, ENG, W. K, SCOTT, G. F, XIAOTONG LI, CARR, S. A, JOHNSON, R. K, WINKLER, J. D, ZHOU, B.-B. S, YUAN ZHU, MATTERN, M. R, MISHRA, R, HURLE, M. R, XIAOLONG ZHANG, ANNAN, R. S, QUINN LU, FAUCETTE, L. F

“…In response to DNA damage and replication blocks, cells activate pathways that arrest the cell cycle and induce the transcription of genes that facilitate…”
Get full text

Phosphopeptide Analysis by Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry by Annan, Roland S, Carr, Steven A

“…In this paper we present methods for identifying and sequencing phosphopeptides in simple mixtures, such as HPLC fractions, at the subpicomole level by (+) ion…”
Get full text

T-cell Ubiquitin Ligand Affects Cell Death through a Functional Interaction with Apoptosis-inducing Factor, a Key Factor of Caspase-independent Apoptosis by Collingwood, Therese S., Smirnova, Evgeniya V., Bogush, Marina, Carpino, Nick, Annan, Roland S., Tsygankov, Alexander Y.

“…The lymphoid protein T-cell ubiquitin ligand (TULA)/suppressor of T-cell receptor signaling (Sts)-2 is associated with c-Cbl and ubiquitylated proteins and has…”
Get full text

The catalytic role of INCENP in Aurora B activation and the kinetic mechanism of Aurora B/INCENP by Yang, Jingsong, Zappacosta, Francesca, Annan, Roland S, Nurse, Kelvin, Tummino, Peter J, Copeland, Robert A, Lai, Zhihong

“…Aurora kinases are a family of serine/threonine protein kinases that play essential roles in mitosis and cytokinesis. AurB (Aurora B kinase) has shown a clear…”
Get more information

Discovery of potent, selective sulfonylfuran urea endothelial lipase inhibitors by Goodman, Krista B., Bury, Michael J., Cheung, Mui, Cichy-Knight, Maria A., Dowdell, Sarah E., Dunn, Allison K., Lee, Dennis, Lieby, Jeffrey A., Moore, Michael L., Scherzer, Daryl A., Sha, Deyou, Suarez, Dominic P., Murphy, Dennis J., Harpel, Mark R., Manas, Eric S., McNulty, Dean E., Annan, Roland S., Matico, Rosalie E., Schwartz, Benjamin K., Trill, John J., Sweitzer, Thomas D., Wang, Da-yuan, Keller, Paul M., Krawiec, John A., Jaye, Michael C.

“…A series of potent, selective sulfonylfuran urea endothelial lipase inhibitors is reported. Mechanism of action studies are also discussed. Endothelial lipase…”
Get full text

A Photoaffinity‐Based Fragment‐Screening Platform for Efficient Identification of Protein Ligands by Grant, Emma K., Fallon, David J., Hann, Michael M., Fantom, Ken G. M., Quinn, Chad, Zappacosta, Francesca, Annan, Roland S., Chung, Chun‐wa, Bamborough, Paul, Dixon, David P., Stacey, Peter, House, David, Patel, Vipulkumar K., Tomkinson, Nicholas C. O., Bush, Jacob T.

“…Advances in genomic analyses enable the identification of new proteins that are associated with disease. To validate these targets, tool molecules are required…”
Get full text

Examination of micro-tip reversed-phase liquid chromatographic extraction of peptide pools for mass spectrometric analysis by Erdjument-Bromage, Hediye, Lui, Mary, Lacomis, Lynne, Grewal, Anita, Annan, Roland S, McNulty, Dean E, Carr, Steven A, Tempst, Paul

“…Mass spectrometry occupies a central position in most current protein identification schemes. So-called `mass fingerprinting' techniques rely on composite mass…”
Get full text