Expression of cytokeratin 20 in thyroid carcinomas and peripheral blood detected by reverse transcription polymerase chain reaction

Expression of cytokeratin 20 in thyroid carcinomas and peripheral blood detected by reverse transcription polymerase chain reaction

We investigated a nested reverse transcriptase polymerase chain reaction (RT-PCR) system to detect CK20 mRNA in thyroid carcinomas, benign thyroid diseases and peripheral blood to improve diagnosis of thyroid carcinoma and to detect disseminated tumour cells. Frozen tissue samples of 46 thyroid carc...

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Bibliographic Details
Published in:British journal of cancer Vol. 82; no. 1; pp. 157 - 160
Main Authors: WEBER, T, LACROIX, J, WEITZ, J, AMNAN, K, MAGENER, A, HÖLTING, T, KLAR, E, HERFARTH, C, VON KNEBEL DOEBERITZ, M
Format: Journal Article
Language:English
Published: Basingstoke Nature Publishing Group 01-01-2000
Subjects:
Adenocarcinoma, Follicular - chemistry
Adenoma - blood
Biological and medical sciences
Biomarkers, Tumor - analysis
Biomarkers, Tumor - blood
Blotting, Northern
Cancer research
Carcinoma, Medullary - chemistry
Cytokeratin
Goiter - blood
Humans
Intermediate Filament Proteins - analysis
Intermediate Filament Proteins - blood
Investigative techniques, diagnostic techniques (general aspects)
Keratin-20
Medical research
Medical sciences
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Polymerase chain reaction
Regular
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
RNA, Messenger - blood
Sensitivity and Specificity
Surgery
Thyroid cancer
Thyroid diseases
Thyroid Neoplasms - blood
Thyroid Neoplasms - chemistry
Thyroiditis, Autoimmune - blood
Tumor Cells, Cultured
Endocrinopathy
Performance evaluation
Human
Cytokeratin
Carcinoma
Thyroid diseases
Reverse transcription
Thyroid gland
Malignant tumor
Blood
Polymerase chain reaction
Tissue
Diagnosis
Technique
Molecular biology
Tumor cell
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Summary:We investigated a nested reverse transcriptase polymerase chain reaction (RT-PCR) system to detect CK20 mRNA in thyroid carcinomas, benign thyroid diseases and peripheral blood to improve diagnosis of thyroid carcinoma and to detect disseminated tumour cells. Frozen tissue samples of 46 thyroid carcinomas and 30 benign thyroid diseases (14 multinodular goiters, 14 follicular adenomas, two Hashimoto's thyroiditis) were obtained intraoperatively. Preoperative blood samples were drawn from 31 patients with thyroid cancer, nine patients with benign thyroid disorders and 20 healthy volunteers. Nine out of nine medullary, 9/12 follicular, 7/19 papillary and 2/6 anaplastic carcinomas expressed CK20 transcripts. CK20 mRNA was undetectable in 30 tissue sections of benign thyroid diseases. Circulating tumour cells were found in the blood of 3/8 patients with medullary carcinoma, 2/8 patients with follicular carcinoma, 2/11 patients with papillary carcinoma and 1/4 patients with an anaplastic carcinoma. Nine blood samples of patients with benign thyroid diseases and 20 healthy volunteers tested negative. For the first time CK20 mRNA could be detected in tissue sections of thyroid carcinomas and peripheral blood samples of patients with thyroid cancer. It was not detectable in benign thyroid diseases. Our results therefore strongly suggest that CK20 RT-PCR assays may improve the diagnosis of thyroid carcinoma and is able to detect circulating tumour cells in peripheral blood of thyroid carcinoma patients.
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ISSN:0007-0920
1532-1827
DOI:10.1054/bjoc.1999.0893