Spectral analysis and bioactive profiling of hot methanolic extracts from Phoenix dactylifera seeds: Antibacterial efficacy and in vitro cytotoxicity insights

Phoenix dactylifera, commonly called date palm, has great importance as a fruiting plant. The hot methanolic extract of date seeds (HMEDSE), was further fractionated into three fractions (F1, F2, and F3) through column chromatography. The three fractions were composed of various bioactive constituen...

Full description

Saved in:

Bibliographic Details
Published in:	Notulae botanicae Horti agrobotanici Cluj-Napoca Vol. 52; no. 2; p. 13600
Main Authors:	MONI, Sivakumar S., MOHAN, Syam, ALAM, Mohammad F., ELMOBARK, Mohamed E., JABEEN, Aamena, SABEI, Fahad Y., REHMAN, Zia ur, ALAM, Md Shamsher, ALOWAYNI, Abdul M.H.M., ASIRI, Ahmed M.O., HASSAN, Dalin A., ABUBAKER MOHAMED, Mawada, ABDALLAH, Hana F., SEETHARAMAN, Shanmuganathan
Format:	Journal Article
Language:	English
Published:	AcademicPres 12-06-2024
Subjects:	anti-bacterial effect hot methanolic extract human pathogenic bacteria in vitro cytotoxicity MCF-7 breast cancer cells Phoenix dactylifera
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Phoenix dactylifera, commonly called date palm, has great importance as a fruiting plant. The hot methanolic extract of date seeds (HMEDSE), was further fractionated into three fractions (F1, F2, and F3) through column chromatography. The three fractions were composed of various bioactive constituents which was analysed through GC-MS and FT-IR analysis. The results revealed remarkable antibacterial properties of crude HMEDSE against various pathogenic microorganisms affecting humans. The spectrum of activity of HMEDSE against various human pathogenic bacteria showed the following sequence based on its efficacy, Escherichia coli (17.6 ± 2.5 mm), Klebsiella pneumoniae (16.3 ± 2.5 mm), Staphylococcus aureus (16.3 ± 1.5 mm), Streptococcus pyogenes (15 ± 2.6 mm), Pseudomonas aeruginosa (15 ± 2 mm), and lastly, Bacillus subtilis (14.3 ± 2 mm). Furthermore, HMEDSE exhibited cytotoxicity, with an IC50 of 73.5 ± 0.5 µg/mL against MCF-7 ATCC breast cancer cells, leading to gradual apoptosis.
ISSN:	0255-965X 1842-4309
DOI:	10.15835/nbha52213600