ALTERATIONS IN IMMUNOGLOBULIN SYNTHESIS DURING ADRIAMYCIN-INDUCED APOPTOSIS IN MOUSE HYBRIDOMA CELLS

ALTERATIONS IN IMMUNOGLOBULIN SYNTHESIS DURING ADRIAMYCIN-INDUCED APOPTOSIS IN MOUSE HYBRIDOMA CELLS

Changes in the immunoglobulin synthesis in mouse hybridoma 1F7cell line following exposure to the anticancer drug adriamycin were studied. It was shown that adriamycin in concentrations up to 0.1μg/ml causes dose-dependent reversible enhancement of immunoglobulin heavy γ2bchain synthesis, and to a l...

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Bibliographic Details
Published in:Cell biology international Vol. 21; no. 2; pp. 69 - 74
Main Authors: MELIKSETIAN, M.B., DAVTYAN, T.K., CHIRKOWA, I.V., ALEXANYAN, YU.T., IGNATOVA, T.N.
Format: Journal Article
Language:English
Published: Oxford, UK Elsevier Ltd 01-02-1997
Blackwell Publishing Ltd
Subjects:
adriamycin
Animals
Antibody Formation - drug effects
apoptosis
Apoptosis - drug effects
Cell Cycle - drug effects
DNA Fragmentation - drug effects
DNA-Binding Proteins - metabolism
Doxorubicin - pharmacology
Gene Expression - drug effects
Histones - metabolism
Hybridomas
immunoglobulin
Immunoglobulin G - biosynthesis
Mice
mouse hybridoma
Nuclear Proteins - metabolism
RNA, Messenger - genetics
Time Factors
apoptosis
immunoglobulin
adriamycin
mouse hybridoma
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Summary:Changes in the immunoglobulin synthesis in mouse hybridoma 1F7cell line following exposure to the anticancer drug adriamycin were studied. It was shown that adriamycin in concentrations up to 0.1μg/ml causes dose-dependent reversible enhancement of immunoglobulin heavy γ2bchain synthesis, and to a lesser extent, κ light chain synthesis. Enhancement of γ2bheavy chain synthesis accompanies an increase in Ig γ2bmRNA levels. Enhancement of DNA-binding activity of another B-cell specific protein, octamer transcription factor Oct-2 was also observed. Cell cycle analysis revealed accumulation of the ADR-treated cells in G2/M phase of cell cycle. These changes in hybridoma cells preceded the onset of internucleosomal DNA fragmentation and appearance of morphological characteristics of apoptosis. These findings suggest, that differentiation-related processes occur during the latent phase of apoptosis induced by adria-mycin in mouse hybridoma cells.
Bibliography:istex:A4C963A249A2F484B171AC1654F648D90647E1F6
ArticleID:CBIN569
ark:/67375/WNG-HK6Z113T-7
Present address: Cancer Center, University of Illinois at Chicago, IL 60607‐7173, U.S.A.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1065-6995
1095-8355
DOI:10.1006/cbir.1996.0116