Search Results - "AKANUMA, Satoshi"

Characterization of Reconstructed Ancestral Proteins Suggests a Change in Temperature of the Ancient Biosphere by Akanuma, Satoshi

“…Understanding the evolution of ancestral life, and especially the ability of some organisms to flourish in the variable environments experienced in Earth's…”
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Reconstruction and Characterization of Thermally Stable and Catalytically Active Proteins Comprising an Alphabet of ~ 13 Amino Acids by Kimura, Madoka, Akanuma, Satoshi

“…While extant organisms synthesize proteins using approximately 20 kinds of genetically coded amino acids, the earliest protein synthesis system is likely to…”
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Comprehensive mutagenesis to identify amino acid residues contributing to the difference in thermostability between two originally thermostable ancestral proteins by Akanuma, Satoshi, Yamaguchi, Minako, Yamagishi, Akihiko

“…Further improvement of the thermostability of inherently thermostable proteins is an attractive challenge because more thermostable proteins are industrially…”
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Comprehensive mutagenesis to identify amino acid residues contributing to the difference in thermostability between two originally thermostable ancestral proteins by Satoshi Akanuma, Minako Yamaguchi, Akihiko Yamagishi

“…Further improvement of the thermostability of inherently thermostable proteins is an attractive challenge because more thermostable proteins are industrially…”
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Comparative analysis of reconstructed ancestral proteins with their extant counterparts suggests primitive life had an alkaline habitat by Fujikawa, Takayuki, Sasamoto, Takahiro, Zhao, Fangzheng, Yamagishi, Akihiko, Akanuma, Satoshi

“…To understand the origin and early evolution of life it is crucial to establish characteristics of the primordial environment that facilitated the emergence…”
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Reconstructed ancestral enzymes suggest long-term cooling of Earth’s photic zone since the Archean by Garcia, Amanda K., Schopf, J. William, Yokobori, Shin-ichi, Akanuma, Satoshi, Yamagishi, Akihiko

“…Paleotemperatures inferred from the isotopic compositions (δ18O and δ30Si) of marine cherts suggest that Earth’s oceans cooled from 70 ± 15 °C in the Archean…”
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Comprehensive reduction of amino acid set in a protein suggests the importance of prebiotic amino acids for stable proteins by Shibue, Rei, Sasamoto, Takahiro, Shimada, Masami, Zhang, Bowen, Yamagishi, Akihiko, Akanuma, Satoshi

“…Modern organisms commonly use the same set of 20 genetically coded amino acids for protein synthesis with very few exceptions. However, earlier protein…”
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Experimental evidence for the thermophilicity of ancestral life by Akanuma, Satoshi, Nakajima, Yoshiki, Yokobori, Shin-ichi, Kimura, Mitsuo, Nemoto, Naoki, Mase, Tomoko, Miyazono, Ken-ichi, Tanokura, Masaru, Yamagishi, Akihiko

“…Theoretical studies have focused on the environmental temperature of the universal common ancestor of life with conflicting conclusions. Here we provide…”
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Robustness of predictions of extremely thermally stable proteins in ancient organisms by Akanuma, Satoshi, Yokobori, Shin-ichi, Nakajima, Yoshiki, Bessho, Mizumo, Yamagishi, Akihiko

“…A number of studies have addressed the environmental temperatures experienced by ancient life. Computational studies using a nonhomogeneous evolution model…”
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Characterization of a thermostable mutant of Agaricus brasiliensis laccase created by phylogeny-based design by Hamuro, Yuhi, Tajima, Katsuya, Matsumoto-Akanuma, Akiko, Sakamoto, Sayaka, Furukawa, Ryutaro, Yamagishi, Akihiko, Ohno, Naohito, Akanuma, Satoshi

“…Laccases are enzymes that oxidize various aromatic compounds, and therefore they have attracted much attention from the standpoints of medical and industrial…”
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Addition of negatively charged residues can reverse the decrease in the solubility of an acidic protein caused by an artificially introduced non-polar surface patch by Yagi, Sota, Akanuma, Satoshi, Yamagishi, Akihiko

“…A non-polar patch on the surface of a protein can cause a reduction in the solubility and stability of the protein, and thereby induce aggregation. However, a…”
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Selection of a platinum-binding sequence in a loop of a four-helix bundle protein by Yagi, Sota, Akanuma, Satoshi, Kaji, Asumi, Niiro, Hiroya, Akiyama, Hayato, Uchida, Tatsuya, Yamagishi, Akihiko

“…Protein–metal hybrids are functional materials with various industrial applications. For example, a redox enzyme immobilized on a platinum electrode is a key…”
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De novo design of protein–protein interactions through modification of inter-molecular helix–helix interface residues by Yagi, Sota, Akanuma, Satoshi, Yamagishi, Manami, Uchida, Tatsuya, Yamagishi, Akihiko

“…For de novo design of protein–protein interactions (PPIs), information on the shape and chemical complementarity of their interfaces is generally required…”
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Characterization of the low-temperature activity of Sulfolobus tokodaii glucose-1-dehydrogenase mutants by SUGII, Taisuke, AKANUMA, Satoshi, YAGI, Sota, YAGYU, Kazuki, SHIMODA, Yukiko, YAMAGISHI, Akihiko

“…Thermophilic enzymes are potentially useful for industrial processes because they are generally more stable than are mesophilic or psychrophilic enzymes…”
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Epistasis effects of multiple ancestral-consensus amino acid substitutions on the thermal stability of glycerol kinase from Cellulomonas sp. NT3060 by Fukuda, Yasuhisa, Abe, Asuka, Tamura, Takashi, Kishimoto, Takahide, Sogabe, Atsushi, Akanuma, Satoshi, Yokobori, Shin-ichi, Yamagishi, Akihiko, Imada, Katsumi, Inagaki, Kenji

“…Thermostable variants of the Cellulomonas sp. NT3060 glycerol kinase have been constructed by through the introduction of ancestral-consensus mutations. We…”
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Birth of Archaeal Cells: Molecular Phylogenetic Analyses of G1P Dehydrogenase, G3P Dehydrogenases, and Glycerol Kinase Suggest Derived Features of Archaeal Membranes Having G1P Polar Lipids by Yamagishi, Akihiko, Akanuma, Satoshi, Nakajima, Yoshiki, Yokobori, Shin-ichi

“…Bacteria and Eukarya have cell membranes with sn-glycerol-3-phosphate (G3P), whereas archaeal membranes contain sn-glycerol-1-phosphate (G1P). Determining the…”
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Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose by Takahashi, Hirokazu, Yamazaki, Hiroyuki, Akanuma, Satoshi, Kanahara, Hiroko, Saito, Toshiyuki, Chimuro, Tomoyuki, Kobayashi, Takayoshi, Ohtani, Toshio, Yamamoto, Kimiko, Sugiyama, Shigeru, Kobori, Toshiro

“…We previously reported that multiply-primed rolling circle amplification (MRPCA) using modified random RNA primers can amplify tiny amounts of circular DNA…”
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Methods for reducing nonspecific interaction in antibody–antigen assay via atomic force microscopy by Wakayama, Jun’ichi, Sekiguchi, Hiroshi, Akanuma, Satoshi, Ohtani, Toshio, Sugiyama, Shigeru

“…We developed a method to measure the rupture forces between antibody and antigen by atomic force microscopy (AFM). Previous studies have reported that in the…”
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Mimicking the evolution of a thermally stable monomeric four-helix bundle by fusion of four identical single-helix peptides by Akanuma, Satoshi, Matsuba, Taku, Ueno, Emi, Umeda, Naoki, Yamagishi, Akihiko

“…Internal symmetry is a common feature of the tertiary structures of proteins and protein domains. Probably, because the genes of homo-oligomeric proteins…”
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Combinatorial Mutagenesis to Restrict Amino Acid Usage in an Enzyme to a Reduced Set by Akanuma, Satoshi, Kigawa, Takanori, Yokoyama, Shigeyuki

“…We developed an effective strategy to restrict the amino acid usage in a relatively large protein to a reduced set with conservation of its in vivo function…”
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