Isolation and Characterization of Cytochrome P450-Producing Bacteria from Various Environments

Eight strains of cytochrome P450 (P450)-producing bacteria were isolated from M9 medium containing a P450-inducer as the sole carbon source from the environment. Strains EP1 to EP6 utilizing 2-ethoxyphenol as the sole carbon source were isolated from the soil of a weed-filled field, the water of a p...

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Bibliographic Details
Published in:	Biocontrol Science Vol. 5; no. 2; pp. 111 - 116
Main Authors:	IKATSU, HISAYOSHI, KINO, YASUHIRO, KAWAHARA, NAOKO, ADACHI, MASAKO, MIYOSHI, SHIN-ICHI, TOMOCHIKA, KEN-ICHI, SHINODA, SUMO
Format:	Journal Article
Language:	English
Published:	Osaka-shi The Society for Antibacterial and Antifungal Agents, Japan 2000 Japan Science and Technology Agency
Subjects:	2-Ethoxyphenol 2-Methoxyphenol Bacterial cytochrome P450 Camphor Metyrapone
Online Access:	Get full text
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Summary:	Eight strains of cytochrome P450 (P450)-producing bacteria were isolated from M9 medium containing a P450-inducer as the sole carbon source from the environment. Strains EP1 to EP6 utilizing 2-ethoxyphenol as the sole carbon source were isolated from the soil of a weed-filled field, the water of a paddy field, laboratory effluent, domestic effluent and river water. Strain MP1 utilizing 2-methoxyphenol and strain CP1 utilizing camphor were isolated from oil-polluted soil and the soil of a weed-filled field, respectively. This suggests the distribution of P450-producing bacteria in the environment. Metyrapone (2-methyl-1, 2-di-3-pyridyl-1-propanone) inhibited the growth of P450-producing bacteria on the media containing a P450-inducer as the sole carbon source, strongly suggesting that the P450 is involved in the catabolism of the inducer.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1342-4815 1884-0205
DOI:	10.4265/bio.5.111