Infrared spectroscopy on poly(dG)-poly(dC) DNA at low hydration

Infrared spectroscopy on poly(dG)-poly(dC) DNA at low hydration

Infrared absorption measurements have been made on dry samples of poly(dG)-poly(dC) DNA at various relative humidity and temperatures. The water content, controlled by the relative humidity, reduces as temperature increases and reaches a very low value of ∼0.1 wpn above 120 °C. This minimum water co...

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Bibliographic Details
Published in:Journal of the Physical Society of Japan Vol. 76; no. 2; p. 24009
Main Authors: ABDURAKHMAN, Hasanudin, TAJIRI, Kenji, YOKOI, Hiroyuki, KURODA, Noritaka, MATSUI, Hiroshi, YANAGIMACHI, Takeshi, TANIGUCHI, Masateru, KAWAI, Tomoji, TOYOTA, Naoki
Format: Journal Article
Language:English
Published: Tokyo Institute of Pure and Applied Physics 01-02-2007
Physical Society of Japan
The Physical Society of Japan
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Nucleic acids
molecular vibrations
Hydration
Temperature effect
Molecular vibration
infrared
Absorption spectrum
structure
DNA
Biopolymer
Humidity
water content
Helical structure
Infrared spectrum
Structural analysis
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Summary:Infrared absorption measurements have been made on dry samples of poly(dG)-poly(dC) DNA at various relative humidity and temperatures. The water content, controlled by the relative humidity, reduces as temperature increases and reaches a very low value of ∼0.1 wpn above 120 °C. This minimum water content is maintained when the samples are brought back to room temperature as long as they are kept at the relative humidity of ∼0%. The molecular vibrations, which characterize the backbone structure as well as the base stacking and pairing, indicate that our samples maintain an A-form double helical structure at all the values of water content. The disorder in the base stacking is observed as the result of the decrease of the water content. Additionally the denaturation appears at high temperatures above 100 °C, which reversibly disappears with decreasing temperature.
ISSN:0031-9015
1347-4073
DOI:10.1143/jpsj.76.024009