Search Results - "., Rosli Md. Illias"
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Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production
Published in Critical reviews in biotechnology (17-02-2018)“…The increasing market demand for oligosaccharides has intensified the need for efficient biocatalysts. Glycosyl hydrolases (GHs) are still gaining popularity…”
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2
Optimisation of signal peptide for recombinant protein secretion in bacterial hosts
Published in Applied microbiology and biotechnology (01-05-2013)“…Escherichia coli —the powerhouse for recombinant protein production—is rapidly gaining status as a reliable and efficient host for secretory expression. An…”
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Carbon nanomaterial properties help to enhance xylanase production from recombinant Kluyveromyces lactis through a cell immobilization method
Published in Applied microbiology and biotechnology (01-11-2021)“…Carbon nanomaterials, due to their catalytic activity and high surface area, have potential as cell immobilization supports to increase the production of…”
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4
Enhanced secretion of cyclodextrin glucanotransferase (CGTase) by Lactococcus lactis using heterologous signal peptides and optimization of cultivation conditions
Published in Journal of biotechnology (20-04-2019)“…•Signal peptide G1 may replace signal peptide Usp45 for application in L. lactis.•CGTase secretion improved 4 fold using G1 signal peptide compared to…”
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5
Optimization of the Reaction Parameters of Immobilized Escherichia coli for β‐Cyclodextrin Production
Published in Chemical engineering & technology (01-12-2023)“…Cyclodextrin (CD) is an important substance for chemical, pharmaceutical, and food industries. Conventionally, free Escherichia coli (E. coli) cells expressing…”
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Strategy in Synthesizing Longer-Chain Levan-Type Fructooligosaccharides by Selective Dextran Macromolecular Cross-Linked Bacillus lehensis G1 Endolevanase Aggregate Immobilization
Published in Catalysts (01-09-2024)“…The formation of cross-linked enzyme aggregates (CLEAs) using macromolecular cross-linkers improves substrate accessibility and enhances enzyme retention…”
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Model-aided atpE gene knockout strategy in Escherichia coli for enhanced succinic acid production from glycerol
Published in Journal of biomolecular structure & dynamics (02-08-2016)“…Succinic acid is an important platform chemical with a variety of applications. Model-guided metabolic engineering strategies in Escherichia coli for strain…”
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Model-assisted formate dehydrogenase-O (fdoH) gene knockout for enhanced succinate production in Escherichia coli from glucose and glycerol carbon sources
Published in Journal of biomolecular structure & dynamics (01-11-2016)“…Succinic acid is an important platform chemical that has broad applications and is been listed as one of the top twelve bio-based chemicals produced from…”
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Improved Sugar Recovery of Alkaline Pre-Treated Pineapple Leaf Fibres via Enzymatic Hydrolysis and Its Enzymatic Kinetics
Published in Fermentation (Basel) (01-11-2022)“…The biofibre complex structure in pineapple leaf fibres (PALFs) can be disrupted using the alkaline pre-treatment method with sodium hydroxide (NaOH)…”
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Optimization and characterization of immobilized E. coli for engineered thermostable xylanase excretion and cell viability
Published in Arabian journal of chemistry (01-06-2022)“…There are many parameters that may have influenced the properties of cell during immobilization process. Particularly, the immobilization methods, carrier…”
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Biochemical and Physical Characterization of Immobilized Candida rugosa Lipase on Metal Oxide Hybrid Support
Published in Catalysts (01-08-2022)“…Enzyme immobilization on inorganic materials is gaining more attention with the potential characteristics of high-surface-area-to-volume ratios, increasing the…”
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Rational mutagenesis of cyclodextrin glucanotransferase at the calcium binding regions for enhancement of thermostability
Published in International journal of molecular sciences (01-05-2012)“…Studies related to the engineering of calcium binding sites of CGTase are limited. The calcium binding regions that are known for thermostability function were…”
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Efficient removal of lignin with the maintenance of hemicellulose from kenaf by two-stage pretreatment process
Published in Carbohydrate polymers (2014)“…•A mild pretreatment using combination of alkali–acid (Ca(OH)2–peracetic acid).•High lignin removal with low sugar loss.•The percentage of lignin,…”
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Enhanced secretory production of hemolysin-mediated cyclodextrin glucanotransferase in Escherichia coli by random mutagenesis of the ABC transporter system
Published in Journal of biotechnology (01-12-2010)“…The hemolysin transport system was found to mediate the release of cyclodextrin glucanotransferase (CGTase) into the extracellular medium when it was fused to…”
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effective extracellular protein secretion by an ABC transporter system in Escherichia coli: statistical modeling and optimization of cyclodextrin glucanotransferase secretory production
Published in Journal of industrial microbiology & biotechnology (01-09-2011)“…Direct transport of recombinant protein from cytosol to extracellular medium offers great advantages, such as high specific activity and a simple purification…”
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Highly sustainable cascade pretreatment of low-pressure steam heating and organic acid on pineapple waste biomass for efficient delignification
Published in Fuel (Guildford) (01-08-2022)“…•The cascade pretreatment achieved the maximum lignin reduction of 67.87%.•Optimized cascade pretreatments provide 54.79% glucose and 69.23% xylose yield.•The…”
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Co-immobilization of magnesium precursor and Candida rugosa lipase on alumina via covalent bonding for biodiesel production
Published in Fuel (Guildford) (01-12-2024)“…[Display omitted] •CRL/L-lysine/MA comprising chemical and biological species on a single support.•CRL/L-lysine/MA presented 85.07% of activity recovery of…”
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Site-saturation mutagenesis of mutant l-asparaginase II signal peptide hydrophobic region for improved excretion of cyclodextrin glucanotransferase
Published in Journal of industrial microbiology & biotechnology (01-12-2017)“…The excretion of cyclodextrin glucanotransferase (CGTase) into the culture medium offers significant advantages over cytoplasmic expression. However, the…”
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Effects of electrospun nanofiber fabrications on immobilization of recombinant Escherichia coli for production of xylitol from glucose
Published in Enzyme and microbial technology (01-01-2024)“…A suitable nanofiber sheet was formulated and developed based on its efficacy in the immobilization of recombinant Escherichia coli (E. coli) to enhance…”
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Enhanced excretion of recombinant cyclodextrin glucanotransferase and cell stability of immobilized recombinant Escherichia coli by reducing tryptone concentration
Published in Journal of chemical technology and biotechnology (1986) (01-11-2023)“…Abstract BACKGROUND Recombinant protein excretion has become a mainstream strategy in reducing downstream processing costs. However, recombinant protein…”
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