Glucooligosaccharide Production by Leuconostoc mesenteroides Fermentation with Efficient pH Control, using a Calcium Hydroxidesucrose Solution
95.3% of the sucrose in a feed batch fermentation (300 g/L) was hydrolyzed by Leuconostoc mesenteroides subp. mesenteroides NRRL B-23188 glucansucrase. Further, the glucose of sucrose formed glucooligosaccharides (GOS) of degree of polymerization (DP) over 2, together with 91.6% of the maltose (200...
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| Published in: | Biotechnology and bioprocess engineering pp. 39 - 45 |
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| Main Authors: | , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
한국생물공학회
01-02-2016
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | 95.3% of the sucrose in a feed batch fermentation (300 g/L) was hydrolyzed by Leuconostoc mesenteroides subp. mesenteroides NRRL B-23188 glucansucrase. Further, the glucose of sucrose formed glucooligosaccharides (GOS) of degree of polymerization (DP) over 2, together with 91.6% of the maltose (200 g/L). Lime saccharate (lime sucrate) was used to control the pH during fermentation. The GOS products had DP between 2 and 7. When Streptococcus mutans mutansucrase (0.1 U/mL) reacted with 0.1% sucrose, addition of 0.1 ~ 10% GOS to the mutansucrase reaction digest resulted in a 56 ~ 90% reduction of mutan formation.
GOS also reduced E. coli (72.2%) and Salmonella sp.
(over 40.0%) growth, when 2.5% GOS was used as a single carbon source, compared to growth using glucose.
The calculated glycemic index and glycemic load of GOS was 8 and 1, respectively, based on a 10 g carbohydrate serving. GOS was calculated to have 2.43 kcal/g. After a glucose tolerance test was performed using C57BL/6 mice, we found that mice treated with GOS showed a 59.4% lower increase in plasma glucose than those treated with maltose. KCI Citation Count: 5 |
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| Bibliography: | G704-000785.2016.21.1.003 |
| ISSN: | 1226-8372 1976-3816 |