국내 5 하수처리장에서 E-screen과 Yeast two-hybrid assay에 의한 에스트로겐 활성도와 내분비계장애물질 농도의 평가

국내 5 하수처리장에서 E-screen과 Yeast two-hybrid assay에 의한 에스트로겐 활성도와 내분비계장애물질 농도의 평가

Effluents from sewage treatment plants(STPs) is a potential source of environmental endocrine disrupting compounds(EDCs) which are known to disrupt the endocrine system of animals, including humans and many aquatic organisms. In this study, we investigated the environmental concentrations of 7 targe...

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Published in:Daehan hwan'gyeong gonghag hoeji Vol. 29; no. 10; pp. 1145 - 1153
Main Authors: 이병천(Byung Cheun Lee), 이지호(Ji Ho Lee), 김현영(Hyun Young Kim), Cuong N. Duong, 나진성(Jin Sung Ra), 장남익(Nam Ik Chang), 김현구(Hyun Koo Kim), 김상돈(Sang Don Kim)
Format: Journal Article
Language:Korean
Published: 대한환경공학회 2007
Subjects:
환경공학
하수처리장
E-screen
내분비계장애물질
Sewage Treatment Plant
EDCs
Yeast two-hybrid assay
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Summary:Effluents from sewage treatment plants(STPs) is a potential source of environmental endocrine disrupting compounds(EDCs) which are known to disrupt the endocrine system of animals, including humans and many aquatic organisms. In this study, we investigated the environmental concentrations of 7 target chemicals(nonylphenol, octylphenol, BPA, estrone(E1), $17{\beta}-estradiol(E2),\;17{\alpha}-ethynyl$ estradiol (EE2) and genistein) at 5 STPs and their corresponding estrogenic activities using E-screen and Yeast two-hybrid assay. The average concentrations of 7 compounds detected by gas-chromatography and mass spectrometry appeared at high levels in influents and lower levels in effluents, which suggested the removal mechanisms through sorption by sludge and subsequent biodegradation processes. Amongst, EE2 and genistein were not found in the effluents. The natural estrogens, E1 and E2, which is known to contribute mostly to the estrogenic potency in effluents, showed high removal ratios of 89.0% and 73.1%, respectively. The estrogen equivalent concentrations(EEQ) by E-screen assay in the STPs were 22.4 ng EEQ/L for influent and 1.98 ng EEQ/L for effluent, which account for 91% decrease in estrogenic activity and well matched with the result of Yeast two-hybrid assay. These results showed the dominant estrogenic compounds at 5 STPs are E1 and E2 and represents most of the estrogenic activity by E-screen. 환경 중에 배출되어 인간을 포함하여 척추동물의 내분비계에 영향을 미치는 내분비계장애물질(endocrine disrupting compounds; EDCs)은 하수처리장 방류수가 주요 오염원인 것으로 알려져 있다. 본 연구에서는 국내 5곳 하수처리장에서 7종(nonylphenol, octylphenol, BPA, estrone(E1), $17{\beta}-estradiol(E2),\;17{\alpha}-ethynyl$ estradiol(EE2), genistein)의 내분비계장애물질의 농도를 조사하고, E-screen과 Yeast two-hybrid assay를 이용하여 에스트로겐 활성도(estrogenic activity)를 분석하였다. 하수처리장에서 GC/MS로 분석된 7종의 내분비계장애물질의 농도 결과, 하수처리 공정에서 슬러지 흡착이나 미생물분해로 유입수에 비하여 유출수에서 크게 감소하였다. EE2와 genistein은 방류수에서 분석 검출한계 이하로 나타났으며, 천연호르몬인 E1과 E2는 하수처리로 각각 89.0%, 73.1%의 높은 농도 제거율을 나타내었으며, 방류수 중의 에스트로겐 활성의 대부분을 차지하였다. E-screen assay로 하수처리장 유입수와 유출수의 에스트로겐 활성도를 분석한 결과 22.4와 19.8 ng-EEQ/L로 나타내어 91%의 감소율을 보였으며, Yeast two-hybrid assay에 의한 결과도 크게 감소하는 유사한 경향을 나타냈다. 5곳 하수처리장 방류수의 E-screen 결과, 에스트로겐 활성을 나타내는 주요 기여물질이 E1과 E2인 것으로 나타났다.
Bibliography:KISTI1.1003/JNL.JAKO200708410685125
G704-000098.2007.29.10.001
ISSN:1225-5025
2383-7810