（131）I标记新型小分子肽VP2
本文通过双功能偶联剂5-（三正丁基锡）-3-吡啶甲酸-N-琥珀酰亚胺酯（SPC）将131I标记到小分子融合多肽VP2上,研究了131I标记多肽VP2的体内外稳定性及在正常小鼠体内的代谢与分布。结果表明,该标记药物室温下放置48h后放化纯度仍可达97%,其在小鼠体内可通过胃肠道快速代谢,在甲状腺的摄取较低。用间接标记法得到的131I-SPC-VP2在体内外有良好的稳定性。...
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| Published in: | 同位素 Vol. 29; no. 1; pp. 1 - 5 |
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| Main Author: | |
| Format: | Journal Article |
| Language: | Chinese |
| Published: |
2016
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | 本文通过双功能偶联剂5-（三正丁基锡）-3-吡啶甲酸-N-琥珀酰亚胺酯（SPC）将131I标记到小分子融合多肽VP2上,研究了131I标记多肽VP2的体内外稳定性及在正常小鼠体内的代谢与分布。结果表明,该标记药物室温下放置48h后放化纯度仍可达97%,其在小鼠体内可通过胃肠道快速代谢,在甲状腺的摄取较低。用间接标记法得到的131I-SPC-VP2在体内外有良好的稳定性。 |
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| Bibliography: | 11-2566/TL MA Huan;TANG Yu;YANG Yuan-you;LIU Ning;LIAO Jia-li;YANG Ji-jun;Key Laboratory of Radiation Physics and Technology,Ministry of Education,Institute of NuclearScience and Technology,Sichuan University; Chengdu 610064, China The VP2 peptide specifically binding to vasoactive intestinal polypeptide receptor 1（VPAC1）receptor was radioiodinated with 131 I by bi-functional linker Nsuccinimidyl 5-（tributylstannyl）-3-pyridinecarboxylate（SPC）,and in vitro and in vivo stability of the labeled peptide were evaluated.Mice were injected intravenously with either 1.2×106 Bq 131I-SPC-VP2 or Na131I.Radiochemical purity and biodistribution were studied at various time points.The result showed that purified 131I-SPC-VP2 exhibited radiochemical purity of 97% at room temperature up to 48 h.The tissue uptake of 131I-SPC-VP2 was low after 6hinjection,especially in thyroid.The result indicated the labeled compound had fast clearance and good stability in vivo. 131I; VP2; labeled compound; peptide; biodistribution |
| ISSN: | 1000-7512 |