干旱胁迫下15种丹参miRNAs差异表达分析

干旱胁迫下15种丹参miRNAs差异表达分析

参考已报道的其他植物中响应干旱胁迫的miRNAs，以其中15个miRNAs为研究对象，10％PEG6000胁迫处理的丹参幼苗为材料，采用实时定量PCR技术分析了这些miRNAs在干旱胁迫前后丹参幼苗叶片中的差异性表达。结果表明，在干旱胁迫6h后，丹参体内miR156、miR157、miR166、miR168、miR169、miR319、miR774、miR894、miR2911、miR3462以及miR3626的表达水平表现为不同程度的上调，其中miR157、miR166、miR319、miR774、miR894、miR2911和miR3626表现为显著性上调；miR159、miR167、mi...

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Bibliographic Details
Published in:广东农业科学 Vol. 40; no. 5; pp. 134 - 137
Main Author: 罗书芳 崔浪军 王健 屈生宪
Format: Journal Article
Language:Chinese
Published: 陕西师范大学生命科学学院/教育部药用资源与天然药物化学重点实验室,陕西西安,710062%陕西师范大学生命科学学院/教育部药用资源与天然药物化学重点实验室,陕西西安710062 2013
安康学院农学与生命科学院,陕西安康725000%安康学院农学与生命科学院,陕西安康,725000
Subjects:
RT—PCR
丹参
差异性表达
干旱胁迫
丹参
干旱胁迫
差异性表达
RT-PCR
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Summary:参考已报道的其他植物中响应干旱胁迫的miRNAs，以其中15个miRNAs为研究对象，10％PEG6000胁迫处理的丹参幼苗为材料，采用实时定量PCR技术分析了这些miRNAs在干旱胁迫前后丹参幼苗叶片中的差异性表达。结果表明，在干旱胁迫6h后，丹参体内miR156、miR157、miR166、miR168、miR169、miR319、miR774、miR894、miR2911、miR3462以及miR3626的表达水平表现为不同程度的上调，其中miR157、miR166、miR319、miR774、miR894、miR2911和miR3626表现为显著性上调；miR159、miR167、miR172以及miR408则表现为下调。对这些miRNAs作用的靶基因进行预测和分析发现，它们主要参与植物的新陈代谢、信号传导和生长发育过程，推测miR169和miR3462参与了丹参对于旱胁迫应答过程。
Bibliography:15 candidate miRNAs in Salvia miltiorrhiza seedlings were studied after the herbs treated with 10% PEG6000 for 6 h. The results showed that the expression levels of the 11 miRNAs in drought treated seedlings were up-regulated in comparison with the control seedlings, in which miR157, miR166, miR319, miR774, miR894, miR2911 and miR3626 were up-regulated significantly. However, the expression levels of miR159, miR167, miR172 and miR408 were down-regulated. The analysis on the target genes of these miRNAs revealed that these miRNAs primarily participated in the plant metabolism , signal transduction and growing development, and inferred that miR169 and miR3462 had taken part in the process of drought stress.
44-1267/S
LUO Shu-fang, CUI Lang-jun, WANG Jian, QU Sheng-xian （1.College of Life Science, Shaanxi Normal University/Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Xi＇an 710062, China; 2. College of Agriculture and Life Sciences, Ankang University, Ankang
ISSN:1004-874X