Microcalorimetric Evaluation of the Effect of Kanamycin： An Analysis Based on the Median-Effect Principle
A study of the effect of drug, kanamycin, on the growth metabolism of recombinant Escherichia coli B 1 was carded out by microcalorimeter monitoring of the metabolic activity of treated cells. Power-time curves of growing recombinant Escherichia coli cell suspensions, treated with different kanamyci...
Saved in:
| Published in: | Chinese journal of chemistry Vol. 25; no. 1; pp. 20 - 24 |
|---|---|
| Main Author: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
Weinheim
WILEY-VCH Verlag
2007
WILEY‐VCH Verlag |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | A study of the effect of drug, kanamycin, on the growth metabolism of recombinant Escherichia coli B 1 was carded out by microcalorimeter monitoring of the metabolic activity of treated cells. Power-time curves of growing recombinant Escherichia coli cell suspensions, treated with different kanamycin doses, were recorded. The extent of the effect was evaluated by changes in the slopes of the microcalorimetric curves and the kinetics of the drug action was interpreted from the time at which these changes reached their maximum values and maintained their maximum values. Experimental dose-effect relationships conform to the median-effect principle of the mass-action law： fa/（1-fa）=（D/D50）^m. A plot of y=lg[（fa）^1-1]^-1 versus x=lg D gives the slope m, D50 and R∞. The experimental results revealed that high concentration of kanamycin had an inhibitory effect on the growth of recombinant Escherichia coli B 1 in the lg phase, and had a promoting effect in the stationary period. Moreover, it was demonstrated that microcalorimetry was a reliable method for the detection of modulatory effects in biology. |
|---|---|
| Bibliography: | kanamycin, microcalorimetry, recombinant Escherichia coli 31-1547/O6 R978.12 istex:3F724B96603CCA7DF1CA117C1E9573D614A424D8 ark:/67375/WNG-PK8TT693-5 the National Science and Technology Key Funds - No. 2003DA901A32 the National Natural Science Foundation of China - No. 20476085 ArticleID:CJOC200790011 |
| ISSN: | 1001-604X 1614-7065 |
| DOI: | 10.1002/cjoc.200790011 |