Effects of Maternal Serum on Permeability of Glomerular Endothelial Cell Membrane

The mechanism of injury on the human glomerular endothelial cells （ciGENC） induced by preeclampsia serum was investigated. Concentration of maternal serum sFlt-1 protein was detected by ELISA. Fluorescently-labeled bovine serum albumin infiltrating through lower chamber of Transwell was measured by...

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Published in:	Journal of Huazhong University of Science and Technology. Medical sciences Vol. 31; no. 1; pp. 17 - 20
Main Author:	杜慧刘海意赵君吴媛媛龚洵周琼徐晶晶李宇琪石鑫玮乔福元
Format:	Journal Article
Language:	English
Published:	Heidelberg Huazhong University of Science and Technology 01-02-2011
Subjects:	Adult Cell Membrane Permeability - physiology ELISA法 Endothelial Cells - pathology Endothelial Cells - physiology Female Humans Kidney Glomerulus - cytology Kidney Glomerulus - physiopathology Medicine Medicine & Public Health Pre-Eclampsia - blood Pre-Eclampsia - physiopathology Pregnancy Vascular Endothelial Growth Factor Receptor-1 - blood Vascular Endothelial Growth Factor Receptor-1 - metabolism 产妇内皮细胞渗透性牛血清白蛋白细胞膜肾小球血管内皮生长因子 glomerular endothelial cells preeclampsia serum sFlt-1 VEGF permeability
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Summary:	The mechanism of injury on the human glomerular endothelial cells （ciGENC） induced by preeclampsia serum was investigated. Concentration of maternal serum sFlt-1 protein was detected by ELISA. Fluorescently-labeled bovine serum albumin infiltrating through lower chamber of Transwell was measured by multifunction microplate reader. Morphologic change of ciGENC was observed under inverted phase contrast microscope. The concentration of sflt-1 in preeclampsia groups was significantly increased as compared with control group （P〈0.01）. Permeability in preeclampsia groups was significantly increased as compared with control group （P〈0.01）. By contrast with severe preeclampsia group, the permeability of ciGENC monolayer in mild preeclampsia group was decreased significantly （P〈0.05）. Intervention of exogenous VEGF significantly decreased permeability of ciGENC in preeclampsia groups. It was concluded that sFlt-1 increased ciGENC permeability by damaging integrity of endothelial barrier function.
Bibliography:	preeclampsia; serum; glomerular endothelial cells; permeability; sFlt-1; VEGF The mechanism of injury on the human glomerular endothelial cells （ciGENC） induced by preeclampsia serum was investigated. Concentration of maternal serum sFlt-1 protein was detected by ELISA. Fluorescently-labeled bovine serum albumin infiltrating through lower chamber of Transwell was measured by multifunction microplate reader. Morphologic change of ciGENC was observed under inverted phase contrast microscope. The concentration of sflt-1 in preeclampsia groups was significantly increased as compared with control group （P〈0.01）. Permeability in preeclampsia groups was significantly increased as compared with control group （P〈0.01）. By contrast with severe preeclampsia group, the permeability of ciGENC monolayer in mild preeclampsia group was decreased significantly （P〈0.05）. Intervention of exogenous VEGF significantly decreased permeability of ciGENC in preeclampsia groups. It was concluded that sFlt-1 increased ciGENC permeability by damaging integrity of endothelial barrier function. 42-1679/R Hui DU, Haiyi LIU , Jun ZHAO , Yuanyuan WU , Xun GONG , Qiong ZHOU , jinxing Xu, Yuqi LI , Xinwei SHI , Fuyuan QIAO （Department of Gynaecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China） ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1672-0733 1993-1352
DOI:	10.1007/s11596-011-0143-7