Correlation between Physical Status of Human Papilloma Virus and Cervical Carcinogenesis

The prevalence of human papilloma virus (HPV)-16 in patients with cervical cancer,the physical status of HPV-16 in patients with cervical lesions,and the role of HPV-16 integration in cervi-cal carcinogenesis were investigated.HPV genotyping was performed by using PCR approach with the primer GP5+/G...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of Huazhong University of Science and Technology. Medical sciences Vol. 32; no. 1; pp. 97 - 102
Main Author:	李科珍金鑫方勇王常玉龚妹陈平波刘佳邓东锐艾继辉
Format:	Journal Article
Language:	English
Published:	Heidelberg Huazhong University of Science and Technology 01-02-2012
Subjects:	Adult Aged Biopsy cancer;genotyping;physical Carcinogenesis Cervical cancer Cervix China Female Genotyping human Human papillomavirus Human papillomavirus 16 - genetics Human papillomavirus 16 - isolation & purification Humans Infection Integration Medicine Medicine & Public Health Middle Aged papilloma Papillomavirus Infections - diagnosis Papillomavirus Infections - virology Polymerase chain reaction Precancerous Conditions - diagnosis Precancerous Conditions - virology Primers Prognosis Risk Factors Statistics as Topic status;integration Uterine Cervical Neoplasms - diagnosis Uterine Cervical Neoplasms - virology virus;cervical China integration physical status human papilloma virus cervical cancer genotyping
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The prevalence of human papilloma virus (HPV)-16 in patients with cervical cancer,the physical status of HPV-16 in patients with cervical lesions,and the role of HPV-16 integration in cervi-cal carcinogenesis were investigated.HPV genotyping was performed by using PCR approach with the primer GP5+/GP6+ and type-specific primer on biopsy specimens taken operatively from 198 women.Multiple PCR was done to detect physical status of HPV-16 in a series of cervical liquid-based cytology samples and biopsy specimens obtained from different cervical lesions with HPV-16 infection,includ-ing 112 specimens with cervical cancer,151 specimens with CINⅠ,246 specimens with CINⅡ and 120 specimens with CINⅢ.The results showed that there were 112 cervical cancer samples (56.57% of total cervical cancer patients) with HPV-16 infection.The frequency of HPV-16 pure integration was 65.18% (73/112),56.57% (47/120),23.58% (58/246) and 7.95% (12/151) in cervical cancer,CINⅢ,CINⅡand CINⅠ patients respectively.In situ hybridization was performed on some paraffin-embedded sections of CINⅡ,CINⅢ and cervical cancer to verify the physical status of HPV-16 infection.Sig-nificant difference was observed between cervical cancer and CINⅠ,CINⅡ,CINⅢ in the frequency of HPV-16 integration (P0.01).It is suggested that HPV-16 is the most prevalent type and is associated with cervical cancer.In the case of HPV-16 infection there are close associations between the severity of cervical lesions and the frequency of HPV-16 integration.The application of testing HPV genotyping and physical status based on detection of HC-Ⅱ HPV DNA would be in favor of predicting the progno-sis of cervical precancerosis and enhancing the screening accuracy of cervical cancer.
Bibliography:	42-1679/R ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1672-0733 1993-1352
DOI:	10.1007/s11596-012-0017-7